猪线粒体抗病毒信号蛋白MAVS对口蹄疫病毒感染宿主的影响
发布时间：2018-09-08 16:46
【摘要】：采用构建猪源MAVS重组质粒pEGFP-MAVS,将其转染PK-15细胞24h后,再感染口蹄疫病毒(MOI=0.1),通过Western blot和real-time PCR检测MAVS融合蛋白表达及对口蹄疫病毒感染宿主细胞的复制和病毒感染滴度。结果显示:重组质粒pEGFP-MAVS能在PK-15细胞中获得表达,并且上调MAVS基因对口蹄疫病毒在宿主细胞中的早期复制有显著抑制作用,病毒的感染滴度也有一定的降低。结果表明:MAVS具有一定的抗病毒作用,此为进一步研究口蹄疫病毒逃逸宿主天然免疫的机制奠定了基础。
[Abstract]:The porcine MAVS recombinant plasmid pEGFP-MAVS, was constructed and transfected into PK-15 cells for 24 hours and then infected with foot-and-mouth disease virus (MOI=0.1). The expression of MAVS fusion protein and the replication and titer of FMDV infected host cells were detected by Western blot and real-time PCR. The results showed that the recombinant plasmid pEGFP-MAVS could be expressed in PK-15 cells, and upregulation of MAVS gene could significantly inhibit the early replication of FMDV in host cells, and the infection titer of FMDV was also decreased to some extent. The results showed that WMAVS had a certain antiviral effect, which laid a foundation for further study on the mechanism of innate immunity of foot-and-mouth disease virus escape host.
【作者单位】： 河北农业大学动物医学院;中国农业科学院兰州兽医研究所;甘肃农业大学动物医学院;
【基金】：国家自然科学基金资助项目(31201943) 国家国际科技合作专项资助项目(2011DFA31830) 国家“863”计划资助项目(2011AA10A211-3)
【分类号】：S852.65
[Abstract]:The porcine MAVS recombinant plasmid pEGFP-MAVS, was constructed and transfected into PK-15 cells for 24 hours and then infected with foot-and-mouth disease virus (MOI=0.1). The expression of MAVS fusion protein and the replication and titer of FMDV infected host cells were detected by Western blot and real-time PCR. The results showed that the recombinant plasmid pEGFP-MAVS could be expressed in PK-15 cells, and upregulation of MAVS gene could significantly inhibit the early replication of FMDV in host cells, and the infection titer of FMDV was also decreased to some extent. The results showed that WMAVS had a certain antiviral effect, which laid a foundation for further study on the mechanism of innate immunity of foot-and-mouth disease virus escape host.
【作者单位】： 河北农业大学动物医学院;中国农业科学院兰州兽医研究所;甘肃农业大学动物医学院;
【基金】：国家自然科学基金资助项目(31201943) 国家国际科技合作专项资助项目(2011DFA31830) 国家“863”计划资助项目(2011AA10A211-3)
【分类号】：S852.65
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