IDO在梅花鹿茸角中的表达及对鹿茸软骨细胞分化的影响
发布时间：2018-10-09 11:41
【摘要】：以梅花鹿茸角为研究对象,通过原位杂交方法检测吲哚胺2,3-双加氧酶1(IDO1)和吲哚胺2,3-双加氧酶2(IDO2)在茸角中的表达。在培养的鹿茸软骨细胞中分别添加IDO1抑制剂1-甲基-L-色氨酸(1-L-MT)和IDO2抑制剂1-甲基-D-色氨酸(1-D-MT),作用24h后,通过荧光定量PCR方法检测软骨细胞分化标志分子Ⅹ型胶原(ColⅩ)表达的变化,进一步研究IDO1和IDO2对鹿茸软骨细胞分化的影响。结果显示:IDO1和IDO2主要在鹿茸真皮层成纤维细胞、间充质细胞和软骨细胞中表达。用浓度分别为200μmol/L和100μmol/L的1-L-MT和1-DMT处理鹿茸软骨细胞24h后,荧光定量PCR结果显示,ColⅩmRNA在鹿茸软骨细胞中的表达量均显著下降。结果表明:当IDO的活性受到抑制时,鹿茸软骨细胞的分化过程也受到明显抑制,IDO可能在梅花鹿鹿茸软骨细胞分化过程中起重要的作用。
[Abstract]:The expression of indoleamine (IDO1) and indole amine (IDO2) in antler horn were detected by in situ hybridization in the antler of Sika deer antler. The results showed that the expression of indoleamine 3-dioxygenase 1 (IDO1) and indoleamine 3-dioxygenase 2 (IDO2) in antler horn were detected by in situ hybridization. Cultured velvet chondrocytes were treated with IDO1 inhibitor 1-methyl-L-tryptophan (1-L-MT) and IDO2 inhibitor 1-methyl-Dtryptophan (1-D-MT) for 24 h. The effect of IDO1 and IDO2 on the differentiation of antler chondrocytes was further studied by detecting the expression of collagen 鈪，
本文编号：2259211
[Abstract]:The expression of indoleamine (IDO1) and indole amine (IDO2) in antler horn were detected by in situ hybridization in the antler of Sika deer antler. The results showed that the expression of indoleamine 3-dioxygenase 1 (IDO1) and indoleamine 3-dioxygenase 2 (IDO2) in antler horn were detected by in situ hybridization. Cultured velvet chondrocytes were treated with IDO1 inhibitor 1-methyl-L-tryptophan (1-L-MT) and IDO2 inhibitor 1-methyl-Dtryptophan (1-D-MT) for 24 h. The effect of IDO1 and IDO2 on the differentiation of antler chondrocytes was further studied by detecting the expression of collagen 鈪，
本文编号：2259211
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