家兔IGF1基因多态性与生长性状的关联分析

发布时间：2018-10-11 19:06

【摘要】：本研究旨在探讨胰岛素样生长因子1(insulin-like growth factor 1,IGF1)基因SNP与家兔生长性状的关联性。采用DNA池、PCR-SSCP方法对家兔IGF1基因的外显子进行多态性检测,结果发现,在家兔IGF1基因的外显子3中检测到1个SNP位点T365C,表现为3种基因型:TT、TC和CC。IGF1基因不同基因型与家兔生长性状的关联分析结果发现,新西兰兔TT基因型个体的初生重、28日龄体重均显著高于TC和CC基因型个体(P0.05);比利时兔TT基因型个体的初生重、90日龄体重和初生至90日龄的平均日增重均显著高于TC和CC基因型个体(P0.05);其余品种的各生长性状指标在该位点均没有达到显著水平(P0.05)。结果提示,IGF1基因可能是影响家兔生长性状的主效基因或与主效基因连锁,T365C位点有望作为提高家兔个体生长性状的分子遗传标记。
[Abstract]:The aim of this study was to investigate the relationship between the SNP of insulin-like growth factor 1 (insulin-like growth factor 1) gene and the growth traits of rabbits. The polymorphism of IGF1 gene exon in rabbits was detected by DNA pool and PCR-SSCP method. A SNP locus T365C was detected in exon 3 of the IGF1 gene in rabbits. The results showed that there were three genotypes: TT,TC and CC.IGF1 genes and their association with the growth traits of rabbits. The birth weight of TT genotype individuals in New Zealand rabbits was significantly higher than that of TC and CC genotype individuals at 28 days old (P0.05), while that of Belgian TT genotype individuals was significantly higher than that of 90 day old individuals and the average daily gain of birth to 90 days old individuals (P0.05). TC and CC genotype individuals (P0.05), the other varieties of growth traits did not reach the level of significance at this locus (P0.05). The results suggest that the IGF1 gene may be a major gene affecting the growth traits of rabbits or be linked to a major gene, and the T365C locus may be a molecular genetic marker to improve the growth traits of rabbits.
【作者单位】：贵州大学动物科学学院高原山地动物遗传育种与繁殖教育部重点实验室贵州省动物遗传育种与繁殖重点实验室;贵州赤水市农牧局;
【基金】：贵州省科技计划项目(黔科合支撑[2016]2512号)
【分类号】：S829.1

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