脱氧雪腐镰刀菌烯醇与玉米赤霉烯酮联合暴露对体外培养鸡脾脏淋巴细胞内环境稳态的影响

发布时间：2018-11-04 19:21

【摘要】：本试验旨在研究脱氧雪腐镰刀菌烯醇(DON)与玉米赤霉烯酮与(ZEA)联合暴露对体外培养鸡脾脏淋巴细胞内环境稳态的影响。分别以0.012 50μg/mL DON+0.006 25μg/mL ZEA、0.050μg/mL DON+0.025μg/mL ZEA、0.2μg/mL DON+0.1μg/mL ZEA、0.8μg/mL DON+0.4μg/mL ZEA对体外培养鸡脾脏淋巴细胞进行联合暴露培养,48 h后测定细胞膜ATP酶(Ca~(2+)-ATP酶、Na~+/K~+-ATP酶)活性以及细胞内pH、Ca~(2+)水平和钙调蛋白(CaM)的mRNA表达水平。同时设不添加毒素的空白对照组。结果表明:添加毒素的各试验组间,细胞内Ca~(2+)水平、CaM mRNA表达水平随毒素浓度的升高而增加,且添加毒素的各试验组均显著或极显著高于空白对照组(P0.05或P0.01)。细胞内pH以及细胞膜Ca~(2+)-ATP酶与Na~+/K~+-ATP酶活性均随毒素浓度的升高而降低,且添加毒素的各试验组均显著或极显著低于空白对照组(P0.05或P0.01)。由此得出,DON、ZEA联合暴露导致体外培养鸡脾脏淋巴细胞内酸化、离子平衡失调等一系列细胞内环境稳态失衡,且呈剂量依赖性。
[Abstract]:The purpose of this study was to investigate the effects of combined exposure of deoxynivalenol (DON) and zearalenone and (ZEA) on the homeostasis of spleen lymphocytes in vitro. 0.012 50 渭 g/mL DON 0.006 25 渭 g/mL ZEA,0.050 渭 g/mL DON 0.025 渭 g/mL ZEA,0.2 渭 g/mL DON 0.1 渭 g/mL ZEA,0.8 渭 g/mL DON 0.4 渭 g/mL ZEA were used for co-exposure culture of chicken splenic lymphocytes in vitro. After 48 h, the activity of ATP (Ca~ (2)-ATP enzyme, Na~ / K- ATP), the level of pH,Ca~ (2) and the mRNA expression of calmodulin (CaM) were measured. At the same time, the blank control group was set up without adding toxin. The results showed that the expression of Ca~ (2), CaM mRNA increased with the increase of toxin concentration. All the test groups were significantly or extremely significantly higher than the blank control group (P0.05 or P0.01). The activities of pH, Ca~ _ (2)-ATP and Na~ / K ~-ATP decreased with the increase of toxin concentration, and the activities of Ca~ _ (2)-ATP and Na~ / K ~-ATP were significantly lower than those of the control group (P0.05 or P0.01). It was concluded that combined exposure to DON,ZEA resulted in a series of intracellular homeostasis such as intracellular acidification ion balance imbalance and so on in vitro in a dose-dependent manner.
【作者单位】：四川农业大学动物医学院;西南科技大学;
【基金】：国家自然科学基金项目(31402269)
【分类号】：S859.87

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