日粮维生素A添加水平与应激对肉仔鸡呼吸道黏膜免疫的影响
发布时间：2018-12-10 11:21
【摘要】：本研究旨在研究不同维生素A添加水平对LPS和NH3免疫应激作用下肉仔鸡呼吸道黏膜免疫的影响,为确定维生素A在维持黏膜完整性的作用机制提供理论依据。试验首先研究了日粮维生素A的确可以影响黏膜免疫功能,之后又考察了LPS的黏膜免疫应激作用。最后研究测定了维生素A在LPS和NH3存在状态下对黏膜免疫功能的影响。试验一,选取1日龄AA肉鸡,按日粮维生素A梯度不同分为4个处理:0 IU/kg、1500 IU/kg、6000 IU/kg、12000 IU/kg。试鸡自由采食与饮水,饲养7d。结果发现:不同维生素A添加量对血清Ig A以及BALF Ig A和黏蛋白的生成产生了显著性差异,当维生素A添加量为0或过高时都会抑制杯状细胞、IgA和黏蛋白的产生。并且对TNF-α、TGF-α、EGFR、IL-13、TGF-β细胞因子的基因表达产生了显著性的影响。研究证明:维生素A的确参与了呼吸道黏膜免疫调控,并能通过影响相关细胞因子的基因表达调控Ig A和黏蛋白的产生。试验二,选取7日龄AA肉鸡,试验组LPS按鸡体重1mg/kg剂量雾化,对照组用相同体积生理盐水雾化,试验鸡暴露2小时,在4/8h之后采样。结果显示:LPS抑制了BALF黏液量的产生,血清和BALF IgA浓度有上升的趋势,且升高了肺组织Ig A的蛋白表达量。结果表明:LPS能够激发呼吸道黏膜免疫应激反应。试验三,根据试验一和试验二的结果,同时结合生产过程中NH3是诱导家禽呼吸道疾病的最主要的因素之一。用LPS和NH3模拟免疫应激,同时添加维生素A,研究维生素A及其LPS和NH3对肉鸡呼吸道黏膜免疫的影响。试验选取1日龄AA肉仔鸡,日粮按维生素A水平不同分为1500IU/kg、6000IU/kg,14日龄时分别用LPS(1mg/kg)和NH3(100ppm)暴露处理2h,在4h之后采样。结果显示:SEM显示氨气、LPS处理呼吸道都造成气道上皮纤毛脱落稀少,NH3和LPS双重刺激造成纤毛脱落较严重,且造成大面积的上皮细胞损伤,日粮添加维生素A可以小面积缓解纤毛的脱落。此外,日粮与NH3对血清Ig A水平、BALF黏蛋白浓度和肺TNF-α、TGF-α、TGF-βmRNA表达水平存在着交互作用,日粮与LPS对BALF黏蛋白浓度、肺组织Ig A蛋白表达和EGFR mRNA表达水平存在着交互作用。以上研究表明:维生素A可通过调控Ig A和黏蛋白的生成,进而影响呼吸道黏膜免疫的功能。维生素A在调控黏膜免疫方面与LPS/NH3存在着一定的交互作用,且维生素A有可能是通过影响细胞因子的基因表达进而缓解LPS/NH3对呼吸道的应激作用,具体的调控机制还需进一步探究。维生素A存在潜在的缓解肉仔鸡呼吸道疾病的作用,对我国AA肉鸡的生产具有一定的指导作用。
[Abstract]:The purpose of this study was to study the effects of different vitamin A supplementation levels on respiratory mucosal immunity of broilers under LPS and NH3 immune stress, and to provide theoretical basis for determining the mechanism of vitamin A in maintaining mucosal integrity. The effect of dietary vitamin A on mucosal immune function was studied firstly, and then the mucosal immune stress of LPS. Finally, the effects of vitamin A on mucosal immune function in the presence of LPS and NH3 were measured. In experiment 1, 1 day old AA broilers were divided into 4 treatments according to dietary vitamin A gradient: 0 IU/kg,1500 IU/kg,6000 IU/kg,12000 IU/kg.. The chickens were fed and drank freely for 7 days. The results showed that there were significant differences in the production of serum Ig A, BALF Ig A and mucin with different vitamin A supplementation, and the production of goblet cells, IgA and mucin was inhibited when the vitamin A concentration was 0 or too high. The gene expression of TNF- 伪, TGF- 伪 and EGFR,IL-13,TGF- 尾 was significantly affected. It has been proved that vitamin A is involved in the regulation of respiratory mucosal immunity and regulates the production of Ig A and mucin by affecting the gene expression of related cytokines. In the second experiment, 7-day-old AA broilers were selected. The LPS of the experimental group was atomized according to the weight of 1mg/kg, while the control group was nebulized with the same volume of normal saline. The chickens were exposed for 2 hours and sampled after 4 / 8 h. The results showed that LPS inhibited the production of BALF mucus, increased serum and BALF IgA concentrations, and increased the expression of Ig A protein in lung tissue. The results showed that LPS could stimulate the respiratory mucosal immune stress response. In experiment 3, according to the results of experiment 1 and experiment 2 and combined with NH3 during production, NH3 is one of the most important factors in inducing respiratory diseases in poultry. The effects of vitamin A and its LPS and NH3 on respiratory mucosal immunity in broilers were studied by simulating immune stress with LPS and NH3 and adding vitamin A at the same time. The diets of 1-day-old AA broilers were divided into 1500 IU / kg / kg ~ (6 000) ~ (-1) ~ (-1) U / kg ~ (-1) / kg ~ (-1) ~ (-1) ~ (-1) ~ (-1) LPS (1mg/kg) and NH3 (100ppm) exposure for 2 h, and then sampled 4 h later. The results showed that ammonia gas was detected by SEM and respiratory tract was treated with LPS. The results showed that the airway epithelial cilia shedding was rare, and the double stimulation of NH3 and LPS caused severe cilia exfoliation, and caused extensive epithelial cell injury. Dietary vitamin A supplementation can alleviate cilia in a small area. In addition, there was interaction between diet and NH3 on serum Ig A level, BALF mucin concentration and lung TNF- 伪, TGF- 伪, TGF- 尾 mRNA expression levels. There is interaction between Ig A protein expression and EGFR mRNA expression in lung tissue. These results suggest that vitamin A can affect the mucosal immune function of respiratory tract by regulating the production of Ig A and mucin. Vitamin A has some interaction with LPS/NH3 in regulating mucosal immunity, and vitamin A may alleviate the stress of LPS/NH3 on respiratory tract by affecting gene expression of cytokines. The specific regulation and control mechanism needs to be further explored. Vitamin A has a potential role in relieving respiratory tract diseases in broilers and has a certain guiding effect on the production of AA broilers in China.
【学位授予单位】：山东农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S831.5
本文编号：2370484
[Abstract]:The purpose of this study was to study the effects of different vitamin A supplementation levels on respiratory mucosal immunity of broilers under LPS and NH3 immune stress, and to provide theoretical basis for determining the mechanism of vitamin A in maintaining mucosal integrity. The effect of dietary vitamin A on mucosal immune function was studied firstly, and then the mucosal immune stress of LPS. Finally, the effects of vitamin A on mucosal immune function in the presence of LPS and NH3 were measured. In experiment 1, 1 day old AA broilers were divided into 4 treatments according to dietary vitamin A gradient: 0 IU/kg,1500 IU/kg,6000 IU/kg,12000 IU/kg.. The chickens were fed and drank freely for 7 days. The results showed that there were significant differences in the production of serum Ig A, BALF Ig A and mucin with different vitamin A supplementation, and the production of goblet cells, IgA and mucin was inhibited when the vitamin A concentration was 0 or too high. The gene expression of TNF- 伪, TGF- 伪 and EGFR,IL-13,TGF- 尾 was significantly affected. It has been proved that vitamin A is involved in the regulation of respiratory mucosal immunity and regulates the production of Ig A and mucin by affecting the gene expression of related cytokines. In the second experiment, 7-day-old AA broilers were selected. The LPS of the experimental group was atomized according to the weight of 1mg/kg, while the control group was nebulized with the same volume of normal saline. The chickens were exposed for 2 hours and sampled after 4 / 8 h. The results showed that LPS inhibited the production of BALF mucus, increased serum and BALF IgA concentrations, and increased the expression of Ig A protein in lung tissue. The results showed that LPS could stimulate the respiratory mucosal immune stress response. In experiment 3, according to the results of experiment 1 and experiment 2 and combined with NH3 during production, NH3 is one of the most important factors in inducing respiratory diseases in poultry. The effects of vitamin A and its LPS and NH3 on respiratory mucosal immunity in broilers were studied by simulating immune stress with LPS and NH3 and adding vitamin A at the same time. The diets of 1-day-old AA broilers were divided into 1500 IU / kg / kg ~ (6 000) ~ (-1) ~ (-1) U / kg ~ (-1) / kg ~ (-1) ~ (-1) ~ (-1) ~ (-1) LPS (1mg/kg) and NH3 (100ppm) exposure for 2 h, and then sampled 4 h later. The results showed that ammonia gas was detected by SEM and respiratory tract was treated with LPS. The results showed that the airway epithelial cilia shedding was rare, and the double stimulation of NH3 and LPS caused severe cilia exfoliation, and caused extensive epithelial cell injury. Dietary vitamin A supplementation can alleviate cilia in a small area. In addition, there was interaction between diet and NH3 on serum Ig A level, BALF mucin concentration and lung TNF- 伪, TGF- 伪, TGF- 尾 mRNA expression levels. There is interaction between Ig A protein expression and EGFR mRNA expression in lung tissue. These results suggest that vitamin A can affect the mucosal immune function of respiratory tract by regulating the production of Ig A and mucin. Vitamin A has some interaction with LPS/NH3 in regulating mucosal immunity, and vitamin A may alleviate the stress of LPS/NH3 on respiratory tract by affecting gene expression of cytokines. The specific regulation and control mechanism needs to be further explored. Vitamin A has a potential role in relieving respiratory tract diseases in broilers and has a certain guiding effect on the production of AA broilers in China.
【学位授予单位】：山东农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S831.5
【参考文献】
相关期刊论文 前1条
1 牛秋红;褚学英;崔小亮;徐丽;刘芳侠;孙琳;;黏蛋白对气道黏液高分泌的抑制及其机制研究[J];生物技术;2011年02期
，本文编号：2370484
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