进境种牛境外免疫对检疫工作的影响及对策

发布时间：2018-12-31 22:14

【摘要】：牛传染性鼻气管炎(IBR)、牛病毒性腹泻(BVD)和牛副流感3型(PI-3)是危害养牛业的三种重要病毒性传染病,他们都能引起牛呼吸道疾病综合征(BRDC),临床表现为高热、食欲下降、精神沉郁、呼吸困难、咳嗽、流涎、眼分泌物增多等,且广泛分布于世界各地,给养牛业造成了巨大的经济损失,我国进出境牛及其产品大多有对这三种疫病检疫的要求。本文测定了在国外接种上述3种疫苗的进口种牛的血清抗体效价,评估了它对入境检验检疫工作的影响,提出了防控上述疾病的建议。研究结果如下:1.IBR血清抗体效价的测定及对IBR防控的建议本文采用ELISA、中和试验和PCR方法,测定了 126头在国外接种灭活IBR疫苗的进口种牛的血清抗体效价和病毒,分析了其对检疫工作的影响,并提出了以接种能区分免疫和自然感染的基因缺失疫苗代替接种灭活常规疫苗的建议。2.BVD血清抗体效价的测定及对BVD防控的建议对126头注射牛病毒性腹泻疫苗的进口牛群血清抗体效价进行了测定,全面分析了其对后续检疫工作的影响,进而提出取消进境种牛境外免疫BVDV疫苗的建议。3.PI-3血清抗体效价的测定及对PI-3防控的建议本文使用ELISA方法,对31头注射牛副流感3型疫苗的进口牛群血清抗体效价进行测定,结合国内外牛副流感3型疫苗的研究进展,建议取消进境种牛境外免疫BPIV3疫苗,并继续研发更加有效的新型疫苗以防控该病的发生和传播。综上所述,本文全面分析了牛传染性鼻气管炎、牛病毒性腹泻及牛副流感3型的防控措施,提出了中肯的建议,为我国从国外进口种牛政策提供了新思路。
[Abstract]:Bovine infectious rhinotracheitis (IBR), bovine viral diarrhea (BVD) and bovine parainfluenza type 3 (PI-3) are three important viral infectious diseases which can cause bovine respiratory disease syndrome (BRDC),). Clinical manifestations include high fever, decreased appetite, depression, dyspnea, coughing, salivation, increased eye discharge, and widespread distribution around the world, causing enormous economic losses to the cattle industry. Most of China's imported and outgoing cattle and their products have quarantine requirements for these three diseases. In this paper, the titer of serum antibody of imported cattle inoculated with the above three kinds of vaccines abroad was determined, its influence on entry inspection and quarantine work was evaluated, and some suggestions for prevention and control of the above diseases were put forward. The results are as follows: determination of 1.IBR serum antibody titer and suggestions for IBR prevention and control. In this paper, the serum antibody titers and viruses of 126 imported cattle inoculated with inactivated IBR vaccine were determined by ELISA, neutralization test and PCR method. Its influence on quarantine work is analyzed. The suggestion of replacing the inactivated routine vaccine with the gene deletion vaccine which can distinguish immunity from natural infection was put forward. The titer of serum antibody of 2.BVD and the prevention and control of BVD for 126 vaccinated bovine viral diarrhea were suggested. The titers of serum antibodies of imported cattle were determined. In this paper, the influence of 3.PI-3 on the subsequent quarantine work was analyzed, and then the suggestion of canceling the BVDV vaccine from imported cattle was put forward. The titer of 3.PI-3 serum antibody and the suggestion of PI-3 prevention and control were determined in this paper, and the ELISA method was used in this paper. The titer of serum antibody against imported bovine parainfluenza type 3 vaccine was determined in 31 cattle injected with bovine parainfluenza 3 vaccine. Combined with the research progress of bovine parainfluenza type 3 vaccine at home and abroad, it was suggested that the BPIV3 vaccine of imported cattle should be cancelled. And continue to develop more effective new vaccines to prevent the occurrence and transmission of the disease. To sum up, the prevention and control measures of bovine infectious rhinotracheitis, bovine viral diarrhea and bovine parainfluenza type 3 are analyzed in this paper.
【学位授予单位】：南京农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S858.23

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