黄羽肉鸡IBV抗体消长规律及免疫基准线的建立

发布时间：2019-02-12 12:02

【摘要】：运用间接ELISA方法对不同日龄(0、21、35、75、126、168、245、315日龄)的黄羽肉鸡采集血清样品,分6个批次,每批次46份样本,共计2 208份血清样品进行鸡传染性支气管炎病毒(IBV)抗体检测,测定抗体消长规律,确定免疫基准线。结果显示:在0日龄时母源抗体为主,抗体滴度在2 400~4 000之间,21日龄时母源抗体基本消退,最低降至159.13,35日龄抗体缓慢上升,到168日龄达到最高峰期,一直维持到315日龄。6个批次鸡除在21日龄差异不显著,其余日龄6个批次间差异显著。抗体均匀度在21日龄最高,0日龄其次,35日龄第三,70日龄第四,245日龄第五,其余日龄差异不大,为最后。研究首次确定黄羽肉鸡抗体基准线,即0日龄在3 053~5 100之间,平均3 391.93;21日龄在93~419之间,平均214.11;35日龄在578~1136之间,平均714.63;70日龄在1 982~3 282之间,平均2 193.53;126日龄在4 465~6 252之间,平均4 428.67;168日龄在4 067~6 290之间,平均5 390.46;245日龄在5 082~6 509之间,平均5 079.98;315日龄在5 198~7 477之间,平均5 281.43。
[Abstract]:Indirect ELISA method was used to detect the (IBV) antibody of chicken infectious bronchitis virus (IBV) in 6 batches (46 samples per batch) of yellow feather broilers of different days (0 ~ 21U ~ (35) ~ (5) ~ (16) 168245315 days old) by using indirect ELISA method, and a total of 2 208 serum samples were collected to detect the (IBV) antibody of chicken infectious bronchitis virus (IBV). The rule of antibody growth and decline was determined, and the immune baseline was determined. The results showed that the maternal antibody was dominant at 0 day old, and the titer of the antibody was between 2 400 and 4 000. At 21 days old, the maternal antibody basically subsided, and the lowest level of maternal antibody increased slowly to 159.13 ~ 35 days old, and reached the peak at 168 days old. There was no significant difference between the 6 batches except 21 days old, but the other 6 batches showed significant differences. The antibody evenness was highest at 21 days old, followed by 0 day old, 35 day third, 70 day fourth, 245 day fifth, and the other days were the last. The antibody baseline of yellow feather broilers was determined for the first time, that is, the age of 0 days was between 3 053 and 5 100, the average age of 3 391.93 days was between 93 and 419, and the average age of 214.1135 days was between 578 and 1136, with an average of 714.63; The age of 70 days was between 1 982 and 3 282, with an average age of 2 193.53126 days in the range of 4 4655 / 6252, an average of 4 428 / 67 / 168 and an average of 4 067 / 6290 and an average of 5 390.46 / 245 / 245 days, respectively, with an average of 5 079.98; The age of 315 days was between 5 198 and 7 477, with an average of 5 281.43.
【作者单位】：广西鸿光农牧有限公司;广西动物疫病预防控制中心;
【基金】：玉林市科学研究与技术开发计划项目(玉市科计201503010)
【分类号】：S858.31

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