延边地区犬瘟热现况研究及基因分型鉴定

发布时间：2019-02-22 17:38

【摘要】：由病毒引起的犬瘟热(canine distemper,CD)是极为严重的一种传染病,可以传播于不同物种之间,对肉食目等数十种动物产生致命性威胁。感染犬瘟热的动物往往因为免疫抑制,继发细菌或其他病毒感染。现已经成为家养宠物、保护野生动物及饲养经济动物重大疫病之一。为了进一步了解延边地区犬瘟热现况,于2014年9月至2016年2月收集延边地区动物医院门诊病例519例,运用胶体金检测法进行诊断,其中阳性病例243例,平均现患率为46.82%。本次调查分析表明患病时间、年龄、免疫情况是影响延边地区犬瘟热现患率的主要因素,性别和品种的差异对犬瘟热现患率影响很小,为该病的进一步研究提供基础数据支持；本次研究发现目前延边地区犬瘟热防控措施有待改善,提出了综合防治措施和建议。根据犬瘟热病毒H基因的突变性,运用半巢式多重PCR的方法对病犬所感染的犬瘟热病毒进行基因分型,共收集病料21例,结果均为Asia-I型,说明延边地区主要流行毒株为Asia-I型。运用PCR法对CDV进行基因分型,可以在1 d内得到结果,具有极高的时效性,可以快速区分患犬瘟热动物的感染来源,鉴别疫苗株和野毒株,同时为犬瘟热的流行病学调查及分子生物学研究提供可靠方法。
[Abstract]:Canine distemper (canine distemper,CD) caused by virus is an extremely serious infectious disease, which can spread between different species and pose a fatal threat to dozens of animals such as carnivorous order. Animals infected with canine distemper are often infected with immunosuppressive, secondary bacteria or other viruses. Now it has become one of the major diseases of domestic pets, protecting wild animals and raising economic animals. In order to further understand the current situation of canine distemper in Yanbian area, 519 outpatients of Yanbian Regional Animal Hospital were collected from September 2014 to February 2016. The diagnosis was carried out by colloidal gold test. 243 cases were positive, and the average prevalence rate was 46.82%. The investigation and analysis showed that the prevalence rate of canine distemper in Yanbian area was mainly affected by the time, age and immunity of the disease. The difference of sex and variety had little effect on the prevalence rate of canine distemper, which provided basic data support for further study of canine distemper. This study found that the prevention and control measures of canine distemper in Yanbian area need to be improved, and put forward comprehensive prevention measures and suggestions. Based on the mutation of canine distemper virus H gene, a semi-nested multiplex PCR method was used to type canine distemper virus infected in dogs. 21 cases of canine distemper virus were collected. The results were all Asia-I type. The results showed that the main epidemic strain in Yanbian area was Asia-I type. The genotyping of CDV by PCR method can obtain the results within 1 day, which has a high time-efficiency. It can quickly distinguish the source of infection of canine distemper animals and identify the vaccine strain and wild strain of Canine distemper. It also provides a reliable method for epidemiological investigation and molecular biology of canine distemper.
【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：S855.3

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