安徽地区禽源沙门氏菌分离鉴定与耐药性研究

发布时间：2019-02-24 13:53

【摘要】：为了解安徽地区禽源沙门氏菌的流行菌株和耐药情况,本研究通过培养特性、镜检、生化试验、PCR和血清型鉴定等,对疑似发病禽的116份病料进行沙门氏菌分离鉴定,并采用Kindy-Bauer法对分离菌株进行耐药性分析。试验共分离到42株禽源沙门氏菌,其中包含5个大群11种血清型。B群30株、A群2株、D群7株、C1群2株、C2群1株,其中以B群鼠伤寒沙门氏菌为优势菌株。42株分离菌对阿米卡星、左氧氟沙星和环丙沙星的耐药率均低于20%,而对氨苄西林、链霉素、呋喃唑酮、大观霉素、复方新诺明和多西环素的耐药率达50%以上,其中对氨苄西林的耐药率达97.62%,且41株分离菌株存在多重耐药。结果表明,目前安徽地区禽源沙门氏菌优势流行株是鼠伤寒沙门氏菌,流行菌株呈现较严重的耐药性。本研究为指导临床用药及禽源沙门氏菌的综合防制提供数据支撑。
[Abstract]:In order to understand the epidemic strains and drug resistance of avian salmonella in Anhui area, 116 samples of suspected poultry were isolated and identified by means of culture characteristics, microscopic examination, biochemical test, PCR and serotype identification. The drug resistance of isolated strains was analyzed by Kindy-Bauer method. A total of 42 strains of avian salmonella were isolated, including 5 groups of 11 serotypes, 30 in group B, 2 in group A, 7 in group D, 2 in group C1 and 1 in group C2. The resistant rates of 42 isolates to amikacin, levofloxacin and ciprofloxacin were lower than 20, but to ampicillin, streptomycin, furazolidone, macromycin, ampicillin, streptomycin, furazolidone and micromycin. The drug resistance rate of compound sulfamine and doxycycline was over 50%, and the resistance rate to ampicillin was 97.62%, and 41 strains were multidrug resistant. The results showed that the dominant epidemic strain of avian salmonella in Anhui area was Salmonella typhimurium. This study provides data support for clinical use and comprehensive control of avian salmonella.
【作者单位】：安徽省农业科学院畜牧兽医研究所;
【基金】：安徽省农业科学院院长青年创新基金项目(15B0414) 国家现代农业产业技术体系项目(CARS-41) 安徽省重点研究与开发计划项目(1704a07020066)
【分类号】：S852.61

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