犬细小病毒SH15株的分离鉴定与全基因组分析

发布时间：2019-04-01 23:39

【摘要】：为了解上海地区犬细小病毒的流行及其变异情况,从疑似犬细小病毒感染的死亡犬采集各种组织,无菌处理病料后同步接种F81细胞,盲传至第5代出现细胞病变,从肠组织中分离出1株病毒。采用电镜进行病毒形态学观察,以及HA、HI和PCR等方法鉴定病毒,结果证实为犬细小病毒,命名为CPV-SH15。该病毒的血凝效价为2~9,病毒TCID_(50)为10~(5.2)/m L,测得病毒编码区基因序列的全长为4 869 bp,与上海分离毒株CPV/CN/SH1/2013的同源性为99.8%,亲缘关系较近,说明上海地区的犬细小病毒的遗传变异并不明显。对其VP2基因的氨基酸序列进行分析,确定该细小病毒属于New CPV-2a亚型。该研究为犬细小病毒遗传进化的进一步研究奠定了基础,从而为犬细小病毒病疫苗的研制以及防控提供参考。
[Abstract]:In order to understand the prevalence and variation of canine parvovirus (DPV) in Shanghai area, various tissues were collected from dead dogs suspected of canine parvovirus infection. After aseptic treatment, F81 cells were inoculated at the same time, and the cells were transmitted blindly to the 5th generation. A strain of virus was isolated from intestinal tissue. The morphology of the virus was observed by electron microscope, and the virus was identified by HA,HI and PCR. The virus was confirmed to be canine parvovirus, named CPV-SH15.. The hemagglutination titer and TCID _ (50) of the virus were 2 and 10 ~ (5.2) / mL, respectively. The full-length sequence of the coding region of the virus was 4 869 bp, and the homology was 99.8% with that of CPV/CN/SH1/2013 isolated from Shanghai. The relative relationship showed that the genetic variation of canine parvovirus in Shanghai was not obvious. The amino acid sequence of the VP2 gene was analyzed and the parvovirus belonged to New CPV-2a subtype. This study lays a foundation for further study on the genetic evolution of canine parvovirus and provides reference for the development and control of canine parvovirus disease vaccine.
【作者单位】：上海交通大学农业与生物学院上海市兽医生物技术重点实验室;上海市宝山区动物疫病预防控制中心;
【基金】：国家自然科学基金资助项目(31672524)
【分类号】：S852.655

【相似文献】