日粮钴添加水平对生长獭兔生产性能、氮代谢性能和毛囊发育的影响
发布时间：2019-06-04 08:51
【摘要】：本课题研究日粮不同钴添加水平对生长獭兔生产性能、代谢性能和毛囊发育的影响。试验选取基线水平一致的生长獭兔200只,随机分为5组,分别饲喂不同钴添加水平(0、0.1、0.4、1.6、6.4mg/kg)的试验日粮,采用单因素试验设计方法研究其对生长獭兔的影响。试验结果如下:(1)在初始体重(IBW)无显著差异的前提下,日粮钴添加水平对试验末期体重(EBW)有显著影响(P0.05),对平均日采食量(ADI)(P0.01)有极显著影响。对平均日增重(ADG)、料重比(F/G)、全净膛率、半净膛率、肉品质、胸腺指数、脾指数、肝指数、肠绒毛高度、隐窝深度、V/C均无显著影响(P0.05),随着日粮钴添加水平的升高,EBW、ADI、半净膛率、胸腺指数、十二指肠绒毛高度均先升高后降低,并且都在0.4 mg/kg时最高。(2)日粮钴添加水平对粪氮(FN)、可消化氮(DN)、DN/食入氮(IN)、沉积氮(RN)/DN、总蛋白和白蛋白均无显著影响(P0.05),对IN、UN、RN、RN/IN有显著影响(P0.05),并且随着日粮钴添加水平的升高,IN、RN和RN/IN先降低后升高,UN显著降低,在钴添加水平为0.4~1.6 mg/kg时达到最大值。日粮钴添加水平对胰蛋白酶活有显著影响(P0.05),在钴添加水平为1.6 mg/kg时最高。日粮钴添加水平对血清尿素含量有显著影响(P0.05),随着日粮钴添加水平的升高,血清尿素先降低后升高,在钴添加水平为1.6 mg/kg时最低。日粮钴添加水平对粪、尿、肌肉、脾脏和肾脏中钴含量有显著影响(P0.05),并且随着日粮钴添加水平的升高,钴含量均显著增加。(3)日粮钴添加水平对獭兔的皮张面积、皮张重量、皮张厚度没有显著影响(P0.05)。随着日粮钴添加水平的升高,皮张重量呈现先增加后降低的趋势,且在钴添加水平为0.4mg/kg时最高。日粮钴添加水平对獭兔的被毛长度、毛囊密度有显著性影响(P0.05),随着日粮钴添加水平的升高,毛囊密度呈现先增加后降低的趋势,钴添加水平为6.4mg/kg时毛囊密度最低可能是因为钴添加量太高导致轻微中毒。分别选取钴添加量为0和6.4 mg/kg的组进行荧光定量分析和Western Blotting技术分析,结果发现日粮钴添加水平对Tyrosinase、BMP2、BMP4有显著性影响(P0.05),并且高钴日粮组比低钴日粮组表达水平都升高,表明Tyrosinase、BMP2、BMP4参与了钴对毛囊的调控,同时AMPK、Wntlob信号通路没有参与钴对毛囊的调控。综合本试验测定指标,生长獭兔日粮钴适宜添加水平为0.4 mg/kg(实际日粮中钴含量为0.60 mg/kg),其中钴添加量为6.4mg/kg时獭兔有轻微中毒症状。
[Abstract]:In this study, the effects of different dietary cobalt supplementation levels on the performance, metabolic performance and hair follicles development of growing Rex rabbits were studied. 200 growing Rex rabbits with the same baseline level were randomly divided into 5 groups and fed with different cobalt supplementation levels (0, 0.1, 0.4, 1.6, 6.4 mg / kg). The effect of single factor design on the growth of Rex rabbits was studied. The results were as follows: (1) on the premise that there was no significant difference in initial body weight (IBW), dietary cobalt supplementation level had a significant effect on body weight (EBW) at the end of the experiment (P 0.05). It had a significant effect on the average daily feed intake (ADI) (P 0.01). There were no significant effects on average daily gain (ADG), feed weight ratio (F 鈮，
本文编号：2492607
[Abstract]:In this study, the effects of different dietary cobalt supplementation levels on the performance, metabolic performance and hair follicles development of growing Rex rabbits were studied. 200 growing Rex rabbits with the same baseline level were randomly divided into 5 groups and fed with different cobalt supplementation levels (0, 0.1, 0.4, 1.6, 6.4 mg / kg). The effect of single factor design on the growth of Rex rabbits was studied. The results were as follows: (1) on the premise that there was no significant difference in initial body weight (IBW), dietary cobalt supplementation level had a significant effect on body weight (EBW) at the end of the experiment (P 0.05). It had a significant effect on the average daily feed intake (ADI) (P 0.01). There were no significant effects on average daily gain (ADG), feed weight ratio (F 鈮，
本文编号：2492607
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