牛心朴子对动物体组织生物化学及血液学影响的研究

发布时间：2021-05-21 00:29

　　牛心朴子属萝藦科植物,广泛分布于中国北方牧区。作为中草药,临床上主要用于抗炎。其活性成分包括半边莲属碱或类生物碱类、黄酮类、多醣、萜烯、脂肪酸、磷脂（类）、氨基酸和维生素等,其中生物碱类是目前研究的主要内容,并且牛心朴子中已明确的生物碱有13种。本研究对牛心朴子中生物碱类粗提物进行了提取,并对其方法进行了探讨；就牛心朴子全草粉碎物不同剂量（12.5%、25%和50%饲料）对家兔的毒性进行了研究,对其体征变化、存活率、血液生化指标、组织器官病理变化进行了观察；以牛心朴子主要生物碱TMPD灌胃大鼠,研究了TMPD不同剂量（38.5mg/kg、77mg/kg、115.5mg/kg、154.8mg/kg和231.8mg/kg体重）对大鼠组织学、血液学及生物化学等生理生化功能的影响。结果显示,通过研究,建立了以盐酸-水-酒精为溶媒的牛心朴子生物碱的提取方法,生物碱的提取率可达0.23%。家兔出现运动障碍、采食量减少、体重减轻,甚至机体衰竭而死亡。血液白细胞增多,贫血,白蛋白减少。肝脏汇管区小叶间隔纤维化、胆管瘀血。肾脏皮质小管空泡化增加。心脏出现退行性空泡状心肌细胞,肺泡扩张,在231.8mg/... 

【文章来源】：中国农业科学院北京市

【文章页数】：111 页

【学位级别】：博士

【文章目录】：
博士学位论文评阅人、答辩委员会签名表
Abstract
摘要
Abbreviation
CHAPTER 1 INTRODUCTION
    1.1 GENERAL INTRODUCTION
    1.2 Biological activities of phenanthroindolizidine alkaloids
    1.3 Biological activities of piperidine alkaloids
    1.4 Methods of extraction alkaloids
    1.5 Histological and biochemical effects of plants containing alkaloids
    1.6 Genotoxic effects of alkaloids
    1.7 Aim of study
CHAPTER 2 EXTRATION OF ALKALOIDS FROM C KOMAROVII
    2.1 INTRODUCTION
    2.2 MATTERIALS AND METHODS
        2.2.1 plant matterial
        2.2.2 solvents
        2.2.3 method of extraction
        2.2.4 method of detection alkaloid in extracting matterial
    2.3 RESULTS AND DISCUSSION
    2.4 CONCLUSION
CHAPTER 3 BIOCHEMICAL, HEMATOLOGICAL ANDHISTOPATHOLOGICAL EFFECT OF C KOMAROVIIAL.ILJINSKI ON RABBITS
    3.1 INTRODUCTION
    3.2 MATERIALS AND METHODS
        3.2.1 Collection,identification and preperation of plants materials
        3.2.2 Animal and experimental design
        3.2.3 Blood and tissue collection
        3.2.4 Biochemical and hematological analysis
        3.2.5 Histopathological analysis
            3.2.5.1 Paraffin section techniques
            3.2.5.2 Hematoxylin and eosin stain technique
        3.2.6 special stain
            3.2.6.1 Trichrmoe stain (Masson)
            3.2.6.2 Gomori(Gordon and Sweat)stains
        3.2.7 Histopathological examination
        3.2.8 Statistical analysis
    3.3 RESULTS
        3.3.1 Clinical signs
        3.3.2 Effects of plants on rabbit food consumption
        3.3.3 Effects of plants on weight of animals
        3.3.4 Biochemical analysis results
        3.3.5 Hematological analysis results
        3.3.6 Histopathological results
    3.4 DISSCUSION
    3.5 CONCLUSION
CHAPTER 4 BIOCHEMICAL,HEMATOLOGICAL ANDHISTOPATHOLOGICAL EFFECT OF 2,2,6,6-TETRAMETHYL-4-PIPERIDONE ON RATS
    4.1 INTRODUCTION
    4.2 MATERIALSAND METHODS
        4.2.1 Chemicals matterial
        4.2.2 Collection and maintenance of animals
        4.2.3 Determination of median lethal dose(LD50)
        4.2.4 Experimental design
        4.2.5 Biochemical and hematological analysis
        4.2.6 Histopathological examination
        4.2.7 Molecular analysis
            4.2.7.1 Animal and sample collection
            4.2.7.2 RNA extraction and RT-PCR
            4.2.7.3 Real time RT-PCR of rats mRNA
        4.2.8 Statistical analysis
    4.3 RESULTS
        4.3.1 Clinical signs
        4.3.2 Effects of TMPD on weight of treated rats
        4.3.3 Biochemical and hematological assay
            4.3.3.1 Effects of TMPD on rats treated for one week
            4.3.3.2 Effects of TMPD on rats treated for two week
        4.3.4 Histopathological results
        4.3.5 Bcl211,Bcl2,cytochrome C and FAAD protein expression
    4.4 DISSCUSION
    4.5 CONCLUSION
CHAPTER 5 GENERAL CONCLUSION
REFERENCE
ACKNOWLEDGEMENT
RESUME



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