The Mutations of Candidate Genes and Their Effects on Reprod

发布时间：2021-07-28 06:39

　　The purpose of this study was to find the molecular markers related with reproduction and milk production traits in two populations consisting dairy cow and water buffalo. Single nucleotide polymorphisms （SNP） on three gene, including FBXL13, ARID4B, and LOC518224 that have beed identified as association with reproduction traits in dairy cows by BovineSNP50 BeadChip in Chinese and British population of dairy cow in previous in this laboratory were again evaluated with reproduction and milk produ... 

【文章来源】：华中农业大学湖北省 211工程院校 教育部直属院校

【文章页数】：50 页

【学位级别】：硕士

【文章目录】：
ABSTRACT
Abbreviations
CHAPTER Ⅰ INTRODUCTION
CHAPTER Ⅱ LITERATURE REVIEW
    2.1 Reproduction Trait
    2.2 Milk Production Trait
    2.3 RFLP
    2.4 SNP Association Studies in Reproduction and Production Trait in Dairy Cow and Water Buffalo
    2.5 Candidate Gene Approach
        2.5.1 FBXL13 (F-box and leucine-rich repeat protein 13) Gene
        2.5.2 ARID4B (AT Rich Interactive Domain 4B) Gene
        2.5.3 LOC518224 Gene
CHAPTER Ⅲ MATERIAL AND METHOD
    3.1 Material Research
        3.1.1 DNA samples
        3.1.2 Equipment and supplies
        3.1.3 Reagents and solutions
    3.2 Method
        3.2.1 Blood sample
        3.2.2 DNA extraction
        3.2.3 SNP detection and genotyping
        3.2.4 Statistical analizes
CHAPTER Ⅳ RESULTS AND DISCUSSION
    4.1 Polymorphism of FBXL13, ARID4B, LOC518224 Candidate Gene in Dairy Cows and Water Buffaloes
        4.1.1 Polymorphism of FBXL13 candidate gene in Dairy Cows
        4.1.2 Polymorphism of FBXL13 candidate gene in Water Buffaloes
        4.1.3 Polymorphism of ARID4B candidate gene in Dairy Cows
        4.1.4 Polymorphism of ARID4B candidate gene in Water Buffaloes
        4.1.5 Polymorphism of LOC518224 candidate gene in Dairy Cows
    4.2 FBXL13 Candidate Gene Effects in Reproduction and Milk Production Traits on Dairy Cows
        4.2.1 Analyze of FBXL13 candidate gene effects in reproduction traits on Dairy Cows
        4.2.2 Analyze of FBXL13 candidate gene effects in milk production traits on Dairy Cows
    4.3 ARID4B Candidate Gene Effects in Reproduction and Milk Production Traits onDairy Cows
        4.3.1 Analyze of ARID4B candidate gene effects in reproduction traits on Dairy Cows
        4.3.2 Analyze of ARID4B candidate gene effects in milk production traits on Dairy Cows
    4.4 LOC518224 Candidate Gene Effects in Reproduction and Milk Production Traitson Dairy Cows
        4.4.1 Analyze of LOC518224 candidate gene effects in reproduction traits on Dairy Cows
        4.4.2 Analyze of LOC518224 candidate gene effects in milk production traits on Dairy Cows
    4.5 FBXL13, ARID4B, LOC518224 Candidate Gene Effects in Reproduction and Milk Production Traits on Water Buffaloes
CHAPTER Ⅴ DISCUSSION
CHAPTER Ⅵ CONCLUSION
REFERENCES
ACKNOWLEDGEMENTS



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