鼻气管鸟杆菌小菌落的分离鉴定

发布时间：2021-11-03 21:01

　　鼻气管鸟杆菌（Omithobacterium rhinotracheale, ORT）是一种与多种禽类,特别是鸡的呼吸道疾病相关的革兰氏阴性菌,广泛分布于世界各地,给禽类产业造成了一定的经济损失。对于该菌的致病机制及流行病学尚缺乏系统的研究,特别是我国禽类的感染和流行情况的研究甚少。本研究从不同地区321份患有临床呼吸道疾病的禽类的气管样品中分离到27株的ORT,其中21株（77.8%）在首次接种时即分离到小菌落变异株（small colony variant, SCV）,经过连续传代,从中挑选出5株稳定的小菌落变异株及其对应的亲代菌株进行了研究。采用API-20NE进行生化鉴定,结果表明5株ORT小菌落变异株的生化反应均为0020000型,而与之相对应的亲代菌株则出现两种不同生化反应结果：ORT-WT3、ORT-WT13、ORT-WT21和ORT-WT28为0020004型,ORT-WT16为0220004型。五株小菌落变异株及亲代菌株的p-半乳糖苷酶检测结果为阳性,亲代菌株氧化酶实验结果为阳性,而小菌落变异株的结果为阴性。体外培养试验表明,小菌落变异株较其和亲代菌株生长速度慢,迟缓... 

【文章来源】：中国农业大学北京市 211工程院校 985工程院校 教育部直属院校

【文章页数】：93 页

【学位级别】：博士

【文章目录】：
摘要
Abstract
Contents
Abbreviation
Index of figures
Index of tables
Introduction
    Ornithobacterium rhinotracheale
        History
        Etiology Classification,Name and Synonyms
        Isolation and Identification
        Growth Requirements
        Morphology and Stainin
        Biochemical idenitifcaiton
        Strain Classificaiton
        Serological
        Molecular
        Experimental Infection
        Mode of Infection
        Pathogenicity
        O.rhinotracheale infections
        Diagnosis
        Serological Examination for Antibody Detection
        Epidemiological Investigations
        Treatment
        Vaccination
    Small colony variants
        History
        Auxotrophic phenotypes of SCVs
        Electron-transport-defective SCVs
        Thymidine-biosynthetic-defective SCVs
        The clinical presentation of SCV infections
        Isolating and identifying SCVs in clinical microbiology laboratory
        The pathogenesis of SCV infections
Virulence in animal models
    Aim of the studies described in this thesis
Material and methods
    Samples collection
    Isolation and identification of ORT-WTs and ORT-SCVs
    PCR and sequence analysis
    Biochemical characteristics
    Bacterial growth curve and enrichment on broth media
    Hemagglutination assay(HA)
    Auxotrophism assay
    Antimicrobial Susceptibility Testing
    Autolysis Assay
    Preparation of ORTs isolates for the assay
    Intracellular Persistence assay
Results
    Isolation of ORT
    Cultivation and morphological characteristics of ORT-WTs and ORT-SCVs
    Biochemical characteristics
    Bacterial Growth Curves
    Hemagglutination assay
    Antimicrobial Susceptibility Testing
    Autolysis Assay
    Intracellular persistence assay
    Phylogenetic analyzes
Discussion
Summary
Conclusion
References
Acknowledgement
Curriculum vitae
    Education and Qualifications
    Employment History
    Academic Papers
    Interests and Activities



本文编号：3474343