内蒙古草原和森林生态系统木霉菌生物多样性研究
发布时间：2022-12-17 14:36
木霉菌是一类常见的真菌,广泛分布于世界各地,在工业和农业中具有显著的经济价值。从内蒙古自治区草原生态系统和森林生态系统采集的土壤中分离出木霉菌,然后对木霉菌种进行鉴定,并对其区域分布、生物多样性及潜在生物防治能力进行评价。主要研究结果如下:1、内蒙木霉菌资源多样性。2014-2016年,从内蒙古草原和森林生态系统共采集土样711份,共分离得到417个木霉菌株,采用形态学和分子鉴定相结合的方法,共鉴定出21个已知木霉种:T.cf.harzianum（205）,T.longibrachiatum（112）,T.afroharzianum（27）,T.koningiopsis（16）,T.asperelloides（13）,T.hamatum（9）,T.rossicum（7）,T.citrinoviride（7）,T.alni（3）,T.konilangbra（2）,T.harzianum（3）,T.saturnisporum（1）,T.crassum（2）,T.velutinum（2）,T.guizhouense（1）,T.gamsii（2）,T.sinuosum,T.atroviridi...
【文章页数】：231 页
【学位级别】：博士
【文章目录】：
摘要
ABSTRACT
LIST OF ABBREVIATIONS
CHAPTER 1 GENERAL INTRODUCTION
1.1 RESEARCH BACKGROUND
1.2 LITERATURE REVIEW
1.2.1 Trichoderma
1.2.2 Taxonomy of Trichoderma
1.2.3 Morphology of Trichoderma
1.2.4 Identification of species based on the analysis of DNA sequences
1.2.5 Internal Transcribed Spacer (ITS)
1.2.6 Roles of Trichoderma as Biological Control Agent
1.2.7 Trichoderma-plant Interactions
1.3 RESEARCH AIM AND OBJECTIVES
1.3.1 Aim
1.4 FLOW CHART OF OUR STUDY
CHAPTER 2 BIODIVERSITY OF THE GENUS HYPOCREA / TRICHODERMA IN DIFFERENT GRASSLAND AND FOREST SOIL ECOSYSTEMS OF INNER MONGOLIA, NORTHERN CHINA
2.1 INTRODUCTION
2.2 MATERIALS AND METHODS
2.2.1 Sample sites and sampling procedure
2.2.2 Isolation of Trichoderma spp
2.2.3 Stock Culture
2.2.4 Morphological Characterization and Growth Rate
2.2.5 Molecular Analysis
2.3 RESULTS
2.3.1 Species identification
2.3.2 Morphological Characterization
2.3.3 Growth rate
2.3.4 Molecular Analysis
2.3.5 Phylogenetic analysis
2.3.6 New species Descriptions
2.3.7 Diversity of Trichoderma/ Hypocrea isolates in Inner Mongolia
2.4 DISCUSSION
CHAPTER 3 DIVERSITY OF TRICHODERMA ISOLATES FROM INNER MONGOLIA SOIL ON BIOLOGICAL CONTROL POTENTIAL
3.1 INTRODUCTION
3.2 MATERIALS AND METHODS
3.2.1 Trichoderma Strains Used
3.2.2 Collection of pathogens
3.2.3 In vitro antagonistic tests
3.2.4 Evaluation on biocontrol and plant growth promotion in vivo
3.2.5 Statistical analysis
3.3 RESULT
3.3.1 In vitro Screening for Antagonistic Potential of Twenty-one Species of Trichoderma against Different Plant Pathogenic Fungi
3.3.2 Screening of indigenous potential antagonistic Trichoderma strains from Inner Mongolia soil against selected phytopathogenic fungi
3.3.3 Analysis of the Antagonistic effect for T.cf harzianum on selected phytopathogenic fungi
3.3.4 Biocontrol efficacy of Trichoderma spp. against wilt of cucumber caused by Fusarium oxysporum f.sp. cucumerinum under In vivo conditions
3.3.5 Effect of treating cucumber seeds with Trichoderma isolates on the severity of Fusarium wilt disease
3.3.6 Biocontrol Potential of Local Trichoderma isolates from Inner Mongolia based on their isolation regions and ecosystem
3.3.7 Biocontrol efficacy of T.cf. harzianum against wilt disease of Cucumber caused by F. oxysporum f.sp. cucumerinum
3.4 DISCUSSION
3.4.1 Biocontrol potential of the isolated strains
CHAPTER 4 DIVERSITY OF LYTIC ENZYMATIC ACTION OF TRICHODERMA ISOLATES SELECTED BASED ON BIOLOGICAL CONTROL PERFORMANCE
4.1 INTRODUCTION
4.2 MATERIALS AND METHODS
4.2.1 Qualitative screening of Extra Cellular Enzyme
4.2.2 Quantitative estimations of Extra Cellular Enzyme
4.3 RESULT
4.3.1 In vitro antifungal activity
4.3.2 Determination of different biocontrol enzymatic activity
4.3.3 Spectrophotometric determination of Enzyme activity of Trichoderma isolates
4.4 DISCUSSIONS
CHAPTER 5 OVERALL CONCLUSION AND RECOMMENDATIONS
5.1 CONCLUSION
5.2 RECOMMENDATION
ACKNOWLEDGEMENTS
RESUME
Publications
REFERENCES
Appendix
本文编号：3720104
【文章页数】：231 页
【学位级别】：博士
【文章目录】：
摘要
ABSTRACT
LIST OF ABBREVIATIONS
CHAPTER 1 GENERAL INTRODUCTION
1.1 RESEARCH BACKGROUND
1.2 LITERATURE REVIEW
1.2.1 Trichoderma
1.2.2 Taxonomy of Trichoderma
1.2.3 Morphology of Trichoderma
1.2.4 Identification of species based on the analysis of DNA sequences
1.2.5 Internal Transcribed Spacer (ITS)
1.2.6 Roles of Trichoderma as Biological Control Agent
1.2.7 Trichoderma-plant Interactions
1.3 RESEARCH AIM AND OBJECTIVES
1.3.1 Aim
1.4 FLOW CHART OF OUR STUDY
CHAPTER 2 BIODIVERSITY OF THE GENUS HYPOCREA / TRICHODERMA IN DIFFERENT GRASSLAND AND FOREST SOIL ECOSYSTEMS OF INNER MONGOLIA, NORTHERN CHINA
2.1 INTRODUCTION
2.2 MATERIALS AND METHODS
2.2.1 Sample sites and sampling procedure
2.2.2 Isolation of Trichoderma spp
2.2.3 Stock Culture
2.2.4 Morphological Characterization and Growth Rate
2.2.5 Molecular Analysis
2.3 RESULTS
2.3.1 Species identification
2.3.2 Morphological Characterization
2.3.3 Growth rate
2.3.4 Molecular Analysis
2.3.5 Phylogenetic analysis
2.3.6 New species Descriptions
2.3.7 Diversity of Trichoderma/ Hypocrea isolates in Inner Mongolia
2.4 DISCUSSION
CHAPTER 3 DIVERSITY OF TRICHODERMA ISOLATES FROM INNER MONGOLIA SOIL ON BIOLOGICAL CONTROL POTENTIAL
3.1 INTRODUCTION
3.2 MATERIALS AND METHODS
3.2.1 Trichoderma Strains Used
3.2.2 Collection of pathogens
3.2.3 In vitro antagonistic tests
3.2.4 Evaluation on biocontrol and plant growth promotion in vivo
3.2.5 Statistical analysis
3.3 RESULT
3.3.1 In vitro Screening for Antagonistic Potential of Twenty-one Species of Trichoderma against Different Plant Pathogenic Fungi
3.3.2 Screening of indigenous potential antagonistic Trichoderma strains from Inner Mongolia soil against selected phytopathogenic fungi
3.3.3 Analysis of the Antagonistic effect for T.cf harzianum on selected phytopathogenic fungi
3.3.4 Biocontrol efficacy of Trichoderma spp. against wilt of cucumber caused by Fusarium oxysporum f.sp. cucumerinum under In vivo conditions
3.3.5 Effect of treating cucumber seeds with Trichoderma isolates on the severity of Fusarium wilt disease
3.3.6 Biocontrol Potential of Local Trichoderma isolates from Inner Mongolia based on their isolation regions and ecosystem
3.3.7 Biocontrol efficacy of T.cf. harzianum against wilt disease of Cucumber caused by F. oxysporum f.sp. cucumerinum
3.4 DISCUSSION
3.4.1 Biocontrol potential of the isolated strains
CHAPTER 4 DIVERSITY OF LYTIC ENZYMATIC ACTION OF TRICHODERMA ISOLATES SELECTED BASED ON BIOLOGICAL CONTROL PERFORMANCE
4.1 INTRODUCTION
4.2 MATERIALS AND METHODS
4.2.1 Qualitative screening of Extra Cellular Enzyme
4.2.2 Quantitative estimations of Extra Cellular Enzyme
4.3 RESULT
4.3.1 In vitro antifungal activity
4.3.2 Determination of different biocontrol enzymatic activity
4.3.3 Spectrophotometric determination of Enzyme activity of Trichoderma isolates
4.4 DISCUSSIONS
CHAPTER 5 OVERALL CONCLUSION AND RECOMMENDATIONS
5.1 CONCLUSION
5.2 RECOMMENDATION
ACKNOWLEDGEMENTS
RESUME
Publications
REFERENCES
Appendix
本文编号：3720104
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