血管性痴呆大鼠模型制作方法的改良

发布时间：2017-12-26 16:01

本文关键词：血管性痴呆大鼠模型制作方法的改良　出处：《中国实验动物学报》2015年06期 　论文类型：期刊论文

【摘要】：目的对血管性痴呆(VD)大鼠模型的造模方法进行改进,使血管性痴呆的实验研究更加科学可靠。方法采用"反复夹闭颈动脉加注射硝普钠降压再加单侧永久结扎颈总动脉法"制备VD大鼠模型,并用脑复康(吡拉西坦)加以模型验证。实验从大鼠行为学和组织病理两个方面评价VD模型。结果实验结果显示:对比假手术组,模型组大鼠定位航行潜伏期延长,空间搜索穿越平台次数减少,海马病理切片显示:细胞数量较少、细胞轮廓模糊皱缩、胞质深染、核不清晰;脑复康阳性药物对照组上述指标明显改善。结论硝普钠注射量为2.0 mg/kg时,手术室温控制在28℃且术后24 h内控制在25℃,大鼠状态稳定,死亡数减少;术中反复阻断三次双侧颈总动脉后直接永久性结扎左侧颈总动脉,可以切断大约三分之一的脑供血,造成慢性脑供血不足状态,更接近VD发病机制。
[Abstract]:Objective to improve the modeling method of rat model of vascular dementia (VD), and to make the experimental research of vascular dementia more scientific and reliable. Methods VD rat model was prepared by repeated clamping of carotid artery plus injection of sodium nitroprusside, and unilateral permanent ligation of common carotid artery. The VD model was evaluated from two aspects of rat behavior and histopathology. The experimental results show that compared with the sham operation group, model group rats positioning navigation latency, reduce the number of search space across the platform, hippocampal slices show that cell number, cell contour fuzzy shrinkage, cytoplasmic hyperchromatic, nuclear is not clear; piracetam positive drug control group were improved. Conclusion sodium nitroprusside injection was 2 mg/kg, the operation temperature control at 28 DEG C and 24 h after operation at 25 C, rats in stable state, to reduce the number of deaths during repeated three times; blocking bilateral common carotid artery after permanent ligation of the left carotid artery, can cut off about 1/3 of the blood supply to the brain, causing chronic cerebral insufficiency, closer to the pathogenesis of VD.
【作者单位】：甘肃省中药药理与毒理学重点实验室;甘肃省中药新产品创制工程实验室;甘肃省中医方药挖掘与创新转化重点实验室;
【基金】：甘肃省中药药理与毒理学重点实验室自主课题(ZDSYS-ZZKJ-2013(B)-004) 国家自然科学基金(81060284)
【分类号】：R749.13;R-332
【正文快照】： gujing120233234@126.comvascular dementia-related experimental studies.血管性痴呆(vascular dementia,VD)是由脑血管病(如脑梗塞、脑出血、皮层下白质的缺血性改变)引起的以记忆、认知功能缺损为主的获得性智能持续性损害。病理变化可见脑实质细胞凋亡,数目减少,脑缩小。

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