家兔腹主动脉瘤模型的建立

发布时间：2017-12-30 18:30

本文关键词：家兔腹主动脉瘤模型的建立　出处：《中南大学》2008年硕士论文　论文类型：学位论文

【摘要】： 目的:为了探讨腹主动脉瘤的发生机理和治疗方法,建立腹主动脉瘤动物模型是不可或缺的研究途径。目前国内尚无制备家兔腹主动脉瘤模型的报道,本研究主要探讨家兔腹主动脉瘤模型的制备方法,为腹主动脉瘤的发生机理和治疗方法的研究做准备。方法:选用健康雄性家兔20只,随机分为4组,2组为灌注弹力蛋白酶溶液实验组,2组为灌注生理盐水对照组,分别于术后2天、7天观察腹主动脉大体形态改变及腹主动脉瘤发生率并取腹主动脉标本行HE染色和VG染色观察组织学改变。结果:灌注弹力蛋白酶组家兔2天后腹主动脉无明显扩大,未形成动脉瘤,灌注弹力蛋白酶组家兔7天后的腹主动脉有明显扩张,平均扩张率为120%,符合腹主动脉瘤的诊断标准,手术成功率80%,成模率100%,HE染色可见动脉壁破坏明显,炎性细胞浸润全层主动脉壁;VG染色可见主动脉中膜弹力蛋白组织严重受损,层次不清,弹力蛋白明显变细,大部分断裂、碎片或缺如,基本符合腹主动脉瘤的病理组织学改变。灌注生理盐水对照组家兔2、7天后均未形成动脉瘤,病理组织学未见明显异常。结论:弹力蛋白酶灌注浸润肾下腹主动脉建立家兔腹主动脉瘤模型是可行的,方法可靠,成功率高,其病理组织学改变基本符合腹主动脉瘤的病理组织学改变。
[Abstract]:Objective: in order to investigate the mechanism and treatment of abdominal aortic aneurysm, the establishment of animal model of abdominal aortic aneurysm is an indispensable research approach. At present, there is no report on the establishment of rabbit abdominal aortic aneurysm model in China. In this study, we studied the preparation of abdominal aortic aneurysm model in rabbits, and prepared for the study of the mechanism and treatment of abdominal aortic aneurysm. Methods: twenty healthy male rabbits were randomly divided into 4 groups (n = 2): the control group (n = 2) were perfused with elastase solution and the control group (n = 2) were perfused with normal saline respectively on the 2nd day after operation. The gross changes of abdominal aorta and the incidence of abdominal aortic aneurysm were observed at 7 days. The histological changes of abdominal aorta were observed by HE staining and VG staining. Results: the abdominal aorta did not dilate and no aneurysm was formed in the perfusion elastase group after 2 days. The abdominal aorta of the rabbits in the perfusion elastase group was dilated obviously after 7 days, and the average dilatation rate was 120%. According to the diagnostic criteria of abdominal aortic aneurysm, the successful rate of operation was 80%, and the rate of model formation was 100%. He staining showed that the arterial wall was destroyed and inflammatory cells infiltrated the whole aortic wall. VG staining showed that the tissue of elastic protein in the aorta was seriously damaged, the layer was not clear, the elastic protein became thinner, most of the fragments were broken, and the fragments were absent. The histopathological changes of abdominal aortic aneurysm were basically consistent with the pathological changes of abdominal aortic aneurysm. No aneurysm was formed in the control group after 2 days of normal saline infusion, and no obvious abnormal histopathology was found in the control group. Conclusion: it is feasible to establish rabbit abdominal aortic aneurysm model by perfusion of elastase into subrenal abdominal aorta. The method is reliable and the success rate is high. The histopathological changes of abdominal aortic aneurysm were basically consistent with the histopathological changes.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R654.3;R-332

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