大鼠骨髓和脂肪间充质干细胞增殖及神经分化能力对比研究

发布时间：2017-12-30 23:38

  本文关键词：大鼠骨髓和脂肪间充质干细胞增殖及神经分化能力对比研究　出处：《新疆医科大学》2014年硕士论文　论文类型：学位论文

  更多相关文章： 骨髓间充质干细胞 脂肪间充质干细胞 细胞分化 神经球 神经元

【摘要】：目的：比较骨髓间充质干细胞和脂肪间充质干细胞的增殖、向神经干细胞诱导及分化为神经元等终末细胞的能力。方法：取同一SD大鼠的骨髓间充质干细胞(Bone marrow mesenchymal stem cells,BMSCs)和脂肪间充质干细胞(Adipose-derived strom cells,ADSCs)体外分离培养,对其形态学、增值速度进行比较。然后将两种间充质干细胞诱导为神经球并分化为神经元,用CCK-8检测神经球的增殖速度,并计算神经球成球率,通过免疫荧光技术比较两种间充质干细胞诱导及分化为终末神经细胞的差异,利用ELISA检测BDNF和NT-3神经营养因子的表达情况。结果：两种间充质干细胞形态上稍有不同,两种细胞均可通过传代达到细胞纯化,ADSCs比BMSCs更快达到诱导所需的细胞纯度。生长曲线上可见ADSCs比BMSCs增值更快。BMSCs和ADSCs均易于用分步法诱导为神经球及分化为终末神经细胞,ADSCs和BMSCs成神经球率分别为21.734±3.46%和15.62±2.37%,ADSCs明显高于BMSCs(P0.05),且成神经球速度快于BMSCs。免疫荧光染色结果显示ADSCs β-tubulinⅢ阳性率(22.18±8.91%)明显高于BMSCs(17.98±11.43%),(P0.05)。ELISA检测结果说明诱导前后ADSCs比BMSCs分泌更多的BDNF和NT-3,差异均有统计学意义(P0.05)。结论：ADSCs较BMSCs取材方便,增殖更快,神经分化率更高,并且能够分泌更多的神经营养因子,在干细胞移植治疗中枢神经系统疾病方面是BMSCs良好的替代来源。
[Abstract]:Objective: to compare the proliferation of bone marrow mesenchymal stem cells and adipose mesenchymal stem cells. The ability of neural stem cells to induce and differentiate into neurons and other terminal cells. Methods: bone marrow mesenchymal stem cells (BMSCs) from the same SD rats were obtained. Bone marrow mesenchymal stem cells. BMSCs) and adipose mesenchymal stem cells (Adipose-derived strom cells) were isolated and cultured in vitro. Then the two kinds of mesenchymal stem cells were induced to be neurospheres and differentiated into neurons. CCK-8 was used to detect the proliferation rate of the neurons and to calculate the spheroidization rate of the two kinds of mesenchymal stem cells. The difference of induction and differentiation of mesenchymal stem cells into terminal nerve cells was compared by immunofluorescence technique. ELISA was used to detect the expression of BDNF and NT-3 neurotrophic factors. Results: the morphology of the two mesenchymal stem cells was slightly different and the two kinds of cells could be purified by subculture. ADSCs can reach the cell purity needed for induction faster than BMSCs. The growth curve shows that ADSCs can increase value faster than BMSCs. BMSCs and ADSCs are easy to be induced by stepwise method. Through the ball and differentiation into terminal nerve cells. The neuron-forming rate of ADSCs and BMSCs were 21.734 卤3.46% and 15.62 卤2.37, respectively, which were significantly higher than those of BMSCs (P 0.05). The positive rate of ADSCs 尾 -tubulin 鈪，

本文编号：1357065