呼吸道合胞病毒培养及其病毒滴度检测方法的建立

发布时间：2017-12-31 22:09

  本文关键词：呼吸道合胞病毒培养及其病毒滴度检测方法的建立　出处：《中国生物制品学杂志》2015年09期 　论文类型：期刊论文

  更多相关文章： 呼吸道合胞病毒 培养条件 病毒滴度

【摘要】：目的确定呼吸道合胞病毒(respiratory syncytial virus,RSV)培养条件,筛选病毒保护剂配方,并建立检测病毒滴度的微量细胞病变方法。方法分别将Hep2、Vero和293R细胞以0.01 MOI接种RSV,选择病毒培养细胞基质;将RSV分别以0.005和0.02 MOI接种Hep2细胞,在4~9 d收获病毒液,检测病毒滴度,确定最佳MOI及病毒收获时间;将6种配方病毒保护剂分别加至病毒液中,反复冻融7次,检测病毒滴度,筛选最佳配方;将病毒分别在33和37℃条件下滴定,第7、9天判定结果,确定微量细胞病变方法的培养温度和判定时间。结果确定病毒培养细胞基质为Hep2细胞,以0.02 MOI RSV接种后,37℃培养7~9 d收获病毒液;配方1(0.1%人血白蛋白)为最佳病毒保护剂配方;微量细胞病变法的实验条件为37℃滴定,7 d判定结果。结论建立了稳定可靠的呼吸道合胞病毒培养及病毒滴度检测方法。
[Abstract]:Objective to determine the culture conditions of respiratory syncytial virus (RSV) and to screen the formula of virus protection agent. Methods Hep2Vero and 293R cells were inoculated with RSV0.01 MOI to select the cell matrix of virus culture. RSV was inoculated with 0. 005 and 0. 02 MOI respectively. The virus solution was harvested at 4 ~ 9 days. The virus titer was detected to determine the best MOI and harvest time. Six kinds of viral protectants were added to the virus solution for 7 times. The titer of virus was detected and the best formula was screened. The virus was titrated at 33 鈩，

本文编号：1361666