骨髓间充质干细胞关节腔注射治疗兔早期类风湿性关节炎的实验研究

发布时间：2018-01-02 21:18

本文关键词：骨髓间充质干细胞关节腔注射治疗兔早期类风湿性关节炎的实验研究　出处：《南京医科大学》2009年硕士论文　论文类型：学位论文

【摘要】：目的：用骨髓间充质干细胞关节腔注射治疗兔早期类风湿性关节炎，探讨其可行性及疗效评价。方法：将60只新西兰大白兔随机分成对照组、类风湿性关节炎组和骨髓间充质干细胞治疗组，每组20只。治疗组在第8周右膝关节腔内注射骨髓间充质干细胞。观察动物的一般情况，TNF-α和IL-1β水平，MRI普通以及增强扫描右膝关节，取膝关节标本行大体及病理观察。结果：所有兔子的一般情况尚好，与类风湿性关节炎组相比，治疗后骨髓间充质干细胞治疗组关节肿胀程度减轻明显，步态渐好转；血清TNF-α和IL-1β水平降低；MRI示低信号区缩小，滑膜的厚度减小，关节软骨厚度接近正常，无明显关节腔积液；大体标本见滑膜有轻度炎性增生，较类风湿性关节炎组轻，关节软骨表面有新生软骨覆盖，呈乳白色，关节面基本平整；病理检查提示有透明样软骨形成，细胞排列整齐，较正常略厚，与软骨相连，，关节面基本平整；滑膜炎症减轻，炎性细胞明显较少。结论：骨髓间充质干细胞在关节腔内可向软骨分化，抑制关节滑膜炎症，为早期类风湿性关节炎患者提供了一种新的有效疗法。
[Abstract]:Objective: to evaluate the feasibility and efficacy of bone marrow mesenchymal stem cell injection in the treatment of early rheumatoid arthritis in rabbits. Methods: sixty New Zealand rabbits were randomly divided into control group, rheumatoid arthritis group and bone marrow mesenchymal stem cell treatment group. 20 rats in each group. Bone marrow mesenchymal stem cells were injected into the right knee joint cavity in the treatment group at the 8th week. The general situation of the animals was observed and the levels of TNF- 伪 and IL-1 尾 were compared with conventional MRI and enhanced scanning of the right knee. The specimens of knee joint were taken for gross and pathological observation. Results: the general condition of all rabbits was good. Compared with rheumatoid arthritis group, bone marrow mesenchymal stem cell treatment group reduced the degree of joint swelling obviously, and the gait gradually improved. The serum levels of TNF- 伪 and IL-1 尾 were decreased. MRI showed that the low signal area was reduced, the thickness of synovial membrane was decreased, the thickness of articular cartilage was close to normal, and there was no obvious effusion in articular cavity. Gross specimens showed mild inflammatory hyperplasia of synovium, lighter than that of rheumatoid arthritis group, the articular cartilage surface was covered with new cartilage, it was milky white, and the articular surface was flat. Pathological examination showed that hyalurondroid cartilage was formed, cells were arranged neatly, slightly thicker than normal, connected with cartilage, and the articular surface was flat. Synovitis is alleviated, inflammatory cell is obviously less. Conclusion: bone marrow mesenchymal stem cells can differentiate into cartilage in articular cavity, inhibit synovitis, and provide a new effective therapy for early rheumatoid arthritis patients.
【学位授予单位】：南京医科大学
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R329

【参考文献】