上焦水饮内停大鼠模型的建立与评价

发布时间：2018-01-03 13:46

本文关键词：上焦水饮内停大鼠模型的建立与评价　出处：《世界中西医结合杂志》2015年06期 　论文类型：期刊论文

【摘要】：目的探索建立符合中医病证特点的上焦水饮内停大鼠模型,并进行生物学特征研究。方法将40只大鼠随机分为空白对照组和模型组,每组20只。模型组给予异丙肾上腺素(ISO)腹部皮下注射,第1天20 mg/kg、第2天10 mg/kg、第3天5 mg/kg、从第4天开始3 mg/kg,1次/d,连续17 d,观察2周,空白对照组腹部皮下注射等量生理盐水。第34天开始模型组行气管插管手术,术后寒冷刺激7 d。结果与空白对照组比较,模型组大鼠肺通透指数明显升高,差异有统计学意义(P0.05);肺泡灌洗液回抽量明显升高,差异有统计学意义(P0.05);肺干湿比明显升高,差异有统计学意义(P0.05);心脏器重量及脏器指数显著升高,差异有统计学意义(P0.01)、肺脏器重量及脏器指数有上升趋势;肌酸激酶(CK)表达明显升高,差异有统计学意义(P0.05);模型组大鼠HE染色显示肺泡壁结构异常,多数肺泡腔内充满粉红色蛋白性液体,呈均质状;部分心肌细胞有嗜酸性变性和(或)坏死的病理改变。结论实验结果表明,上焦水饮内停证动物模型造模方法简单、客观,符合中医病症特点。
[Abstract]:Objective to establish a rat model of Shangjiaoshuiyin in accordance with the characteristics of TCM disease and syndrome, and to study the biological characteristics. Methods Forty rats were randomly divided into blank control group and model group. Each group (n = 20) was subcutaneously injected with isoproterenol ISO (20 mg / kg on day 1, 10 mg / kg on day 2 and 5 mg/kg on day 3). From the 4th day, 3 mg 路kg ~ (-1) 路L ~ (-1) 路d, for 17 days, observed for 2 weeks, the blank control group was subcutaneously injected with the same amount of normal saline, and on the 34th day, the model group underwent tracheal intubation. Results compared with the blank control group, the pulmonary permeability index in the model group was significantly higher than that in the control group (P 0.05). The amount of alveolar lavage fluid redraining was significantly increased, and the difference was statistically significant (P 0.05). The lung dry-wet ratio was significantly higher than that of the control group (P 0.05). Cardiac weight and viscera index increased significantly (P 0.01), and pulmonary organ weight and viscera index increased. The expression of creatine kinase (CK) was significantly increased (P 0.05). In the model group, HE staining showed that the alveolar wall structure was abnormal, and most of the alveolar lumen were filled with pink protein fluid. Some cardiomyocytes have eosinophilic degeneration and / or necrosis pathological changes. Conclusion the experimental results show that the animal model of Shangjiaoshuiyin is simple, objective and accord with the characteristics of TCM disease.
【作者单位】：山东中医药大学;
【基金】：国家重点基础研究发展计划(973计划)(2013CB531803)
【分类号】：R255.8;R-332
【正文快照】： 上焦水饮内停证为一典型的中医证候,临床多表现为呼吸困难,气短喘促,咳吐涎沫,痰量多,舌苔白润或灰腻,舌质胖大,脉沉细[1],与《金匮要略》中“支饮”病证相似,而基础研究领域缺少符合这一中医病证特点的动物模型。为深入探讨上焦水饮内停证本质,并为利水功效的中药药性研究提

【参考文献】