基于厚朴酚对白色念珠菌黏附性及其生物膜形成的影响探讨其抗龋作用

发布时间：2018-01-03 18:23

本文关键词：基于厚朴酚对白色念珠菌黏附性及其生物膜形成的影响探讨其抗龋作用　出处：《首都医科大学学报》2015年06期 　论文类型：期刊论文

【摘要】：目的观察厚朴酚对白色念珠菌黏附性及生物膜形成的影响,以探讨其能否用于治疗白色念珠菌相关龋病。方法运用微孔板法复制白色念珠菌体外黏附及生物膜模型,采用XTT〔2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide〕减低法评价不同浓度厚朴酚对白色念珠菌黏附、生物膜形成的影响。结果厚朴酚对白色念珠菌的黏附具有抑制作用,其作用具有时间及浓度依赖性,62.5 mg/L和125 mg/L厚朴酚可以显著的抑制白色念珠菌的黏附,与阴性对照组相比,差异均有统计学意义(P0.05);实验所选5个浓度的厚朴酚对白色念珠菌的生物膜均有不同程度的抑制作用,其中125 mg/L厚朴酚对生物膜的抑制率达到66.32%。结论厚朴酚可通过抑制白色念珠菌的黏附而有效降低其致病性,并对已形成的生物膜具有明显的抑制作用,提示其作为抗龋药物有良好的应用前景。
[Abstract]:Objective to observe the effect of honokiol on adhesion and biofilm formation of Candida albicans. To explore whether it can be used to treat Candida albicans related caries. Methods XTT(2 was used to replicate Candida albicans adhesion and biofilm model in vitro. 3-bisphon 2-methoxy-4-nitro-5-sulfophenyl-2H-tetrazolium-5-carboxanilide). The adhesion of honokiol to Candida albicans was evaluated by reduction method. Results Magnolol inhibited the adhesion of Candida albicans in a time-and concentration-dependent manner. 62.5 mg/L and 125 mg/L honokiol significantly inhibited the adhesion of Candida albicans, compared with the negative control group, the difference was statistically significant (P 0.05). The 5 concentrations of honokiol could inhibit the biofilm of Candida albicans to varying degrees. The inhibition rate of honokiol on biofilm reached 66.32.Conclusion Magnolol can effectively reduce the pathogenicity of candida albicans by inhibiting its adhesion. The biofilm has obvious inhibitory effect on the formed biofilm, indicating that it has a good application prospect as an anti-caries drug.
【作者单位】：遵义医学院附属口腔医院口腔外科;遵义医学院微生物与免疫学实验室;遵义医学院医学与科技学院;
【基金】：贵州省中医药管理局课题(ky2015-9)~~
【分类号】：R379.4
【正文快照】： 龋病(dental caries)俗称虫牙、蛀牙,是多种因素作用下由口腔常驻菌引起的内源性感染性疾病,其特点是发病率高、分布广,是口腔常见疾病,也是人类最普遍的疾病之一。世界卫生组织已将其与肿瘤和心血管疾病并列为人类三大重点防治疾病。龋洞中的微生物都是以牙菌斑生物膜的形式

【参考文献】