大鼠雪旺细胞培养纯化与鉴定

发布时间：2018-01-04 04:04

本文关键词：大鼠雪旺细胞培养纯化与鉴定　出处：《黑龙江医药科学》2015年06期 　论文类型：期刊论文

【摘要】：目的:通过Wistar大鼠坐骨神经取材,体外预变性与组织块贴壁方法,培养出大量高纯度可以满足临床应用条件的雪旺细胞。方法:取200~250g成年大鼠,脱颈处死,完全浸泡在75%酒精消毒10min,俯卧位取双侧坐骨神经,体外预变性两周,之后加入双酶消化,EGTA试剂纯化雪旺细胞,通过免疫荧光鉴定雪旺细胞纯度。结果:此研究描述一种新颖有效雪旺细胞培养提纯的方法。利用EGTA对雪旺细胞分离比成纤维细胞快的原理提纯,需要大约21d完成。该方法具有操作简单,速度快的优势,比其他先前描述的方法更有效。结论:该实验通过体外预变性获得的雪旺细胞增殖能力强,活性好,易贴壁,而且纯化方法简单易行,在较短的时间内获得高纯度雪旺细胞。
[Abstract]:Objective: to study the methods of predenaturation and tissue mass adherence to the sciatic nerve of Wistar rats in vitro. A large number of Schwann cells with high purity were cultured. Methods: 200g 250g adult rats were killed by removing their necks and completely soaked in 75% alcohol for 10 minutes. The sciatic nerves were predenatured for two weeks in prone position, then the Schwann cells were purified with EGTA reagent. Results: this study described a novel and effective method for the culture and purification of Schwann cells. EGTA was used to purify Schwann cells faster than fibroblasts. It will take about 21 days to complete. This method has the advantages of simple operation and high speed, and is more effective than other methods described earlier. Conclusion: this method has strong proliferative ability through in vitro pre-denaturation. High purity Schwann cells were obtained in a short time.
【作者单位】：佳木斯大学;齐齐哈尔医学院;
【基金】：佳木斯大学研究生创新课题,编号:LM2014-039
【分类号】：R329.2
【正文快照】： 雪旺细胞在周围神经再生中起到决定性作用,是其核心组成。周围神经损伤后,其迅速大量增值,与巨噬细胞协同,分泌神经生长因子[1],同时吞噬变性坏死碎片以及促进轴突再生。因此,雪旺细胞的研究,对周围神经再生起到主导作用[2]。因为成纤维细胞要比雪旺细胞的增殖快,两者的分离提

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