SD大鼠左肺移植模型的建立与改进

发布时间：2018-01-06 05:13

本文关键词：SD大鼠左肺移植模型的建立与改进　出处：《南昌大学学报(医学版)》2015年06期 　论文类型：期刊论文

【摘要】：目的改进大鼠原位左肺移植技术,建立快速、安全、可重复性强的大鼠肺移植动物模型的方法。方法SPF级健康雄性SD大鼠90只,体质量300~350g。其中60只作为供、受体,受体略大于供体,供、受体间体质量差不超过20g,运用三袖套法吻合左肺动、静脉及支气管以建立大鼠左肺移植模型,移植成功后的受体在48h后取肺静脉血进行血气分析(PO2、PCO2);30只为对照组,肺静脉取血进行血气分析。观察记录大鼠肺移植供肺获取、套管及受体移植时间;移植肺血液循环及膨胀情况;移植肺与对照组血气分析结果比较。结果完成大鼠肺移植手术30例,成功27例,失败3例(肺静脉撕裂1例,脱机失败1例,肺动脉扭转1例)。大鼠供肺获取时间为(13.5±2.0)min,供肺套管时间为(15.5±2.5)min;游离大鼠受体左侧肺门部动、静脉及支气管并套线、结扎时间为(19.2±2.0)min;吻合时间为(18.5±4.0)min。在先开放肺动脉血流,接着开放肺静脉血流后,移植的左侧肺叶颜色均匀一致的红润,左肺血流得到充分的灌注,静脉充盈良好,回流畅通;恢复机械通气后,27例移植肺膨胀良好。左肺移植成功48h后,肺移植大鼠右侧肺门夹闭5min后PO2为(7.88±0.84)kPa,PCO2为(6.67±1.09)kPa;对照组大鼠夹闭右侧肺门5min后肺静脉PO2为(8.55±0.97)kPa,PCO2为(6.20±0.88)kPa。2组PO2比较差异有统计学意义(P0.05),PCO2比较差异无统计学意义(P0.05)。结论改进后的肺移植技术可为大鼠肺移植的相关研究提供稳定、可靠及可重复的动物模型。
[Abstract]:Objective to improve the technique of orthotopic left lung transplantation (OLT) in rats and to establish a fast, safe and reproducible animal model of rat lung transplantation. Methods 90 healthy male SD rats of SPF grade were used. 60 of them were used as donors, the recipients were slightly larger than the donors, and the mass difference between the recipients was not more than 20g. The left lung movement was anastomosed with the three-sleeve method. The model of left lung transplantation in rats was established by vein and bronchus. After 48 hours of transplantation, the blood of pulmonary vein was collected for blood gas analysis. 30 rats were used as control group, blood was collected from pulmonary vein for blood gas analysis. The time of lung transplantation donor lung acquisition, cannula and recipient transplantation were observed and recorded. Blood circulation and expansion of transplanted lung; Results there were 27 cases of successful lung transplantation, 3 cases of failure (1 case of pulmonary vein tear, 1 case of weaning failure). In one case of pulmonary torsion, the acquisition time of donor lung was 13.5 卤2.0 min, and the time of cannula of donor lung was 15.5 卤2.5 min. The ligation time of left hilar artery, vein and bronchus of free rat was 19.2 卤2.0 min. The anastomosis time was 18.5 卤4.0 min. After the pulmonary artery was first opened and then the pulmonary vein was opened, the left lobe of the transplanted lung was uniformly red, and the left pulmonary blood flow was fully perfused. The veins were well filled, and the reflux was smooth. The PO2 of the left lung transplantation rats was 7.88 卤0.84kPa after the right hilum was clamped for 5 minutes after 48 hours of left lung transplantation. PCO2 was 6.67 卤1.09kPa. In the control group, the PO2 of pulmonary vein was 8.55 卤0.97 KPA after the right hilum was clipped for 5 minutes. The PO2 of PCO2 group was 6.20 卤0.88kPa.2, the difference was statistically significant (P 0.05). Conclusion the improved lung transplantation technique can provide a stable, reliable and repeatable animal model for the study of lung transplantation in rats.
【作者单位】：南昌大学第一附属医院心胸外科;
【基金】：国家自然科学基金(81360019) 江西省自然科学基金(青年,20132BAB215006)
【分类号】：R655.3;R-332
【正文快照】： 许多像晚期矽肺、晚期肺气肿等终末期肺疾病患者只能通过肺移植来进行有效的治疗[1],而肺移植研究的基础必须建立在稳定的动物模型上。使用SD大鼠来建立肺移植模型,可以很好地模拟人体肺移植,用来进行供肺保存、移植排斥和缺血再灌注损伤等方面的研究,相比其他大型动物的模型

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