脂肪干细胞诱导分化的现状及前景

发布时间：2018-01-06 11:36

本文关键词：脂肪干细胞诱导分化的现状及前景　出处：《中国组织工程研究》2015年06期 　论文类型：期刊论文

【摘要】：背景:脂肪干细胞是由中胚层发育而来的多能干细胞,在特殊的生长因子和环境等诱导培养条件下,可以向不同的谱系分化。目的:详细阐述脂肪干细胞诱导分化的条件及鉴定方法。方法:应用计算机检索万方数据库及PubM ed数据库2005至2014年10年间的文献,中文检索词为"脂肪干细胞,诱导,分化";英文检索词为"adipose derived stem cells,differentiation"。依据纳入排除标准选择37篇文献进行归纳总结。结果与结论:脂肪干细胞在抗坏血酸、胰岛素、地塞米松、转化生长因子β作用下可向软骨细胞分化;成脂诱导液的配方包括3-异丁基-1-甲基黄嘌呤(IBMX)、胰岛素、地塞米松、吲哚美辛;成骨分化常用的诱导剂包含地塞米松或维生素D3、抗坏血酸,β-甘油磷酸钠;碱性成纤维细胞生长因子、表皮生长因子及维生素B27可联合应用诱导脂肪干细胞成神经分化;向心肌细胞分化普遍应用的诱导因子是5-氮杂胞苷;血管内皮生长因子和碱性成纤维细胞生长因子共同作用可以诱导脂肪干细胞向血管内皮细胞分化。随着分子生物学和细胞生物学的迅速发展,脂肪干细胞的分化研究也会更加深入,在目前对脂肪干细胞诱导分化现象观察的基础上,应加强对其内在的分子机制及调控脂肪干细胞可塑性的基因和蛋白的研究。
[Abstract]:Background: adipose stem cells are pluripotent stem cells derived from mesoderm. They are cultured under special conditions such as growth factors and environment. We can differentiate into different lineages. Objective: to elaborate the conditions and identification methods of adipose stem cell induced differentiation. Methods: to search Wanfang database and PubM by computer. Ed database literature for the 10-year period from 2005 to 2014. The key words in Chinese are "adipose stem cells, induction, differentiation"; The key word is "adipose derived stem cells". Differentiation ". Selected 37 articles according to exclusion criteria. Results and conclusions: adipose stem cells in ascorbic acid, insulin, dexamethasone. TGF- 尾 could differentiate into chondrocytes. The formulations of lipopolymers include 3- isobutyl-1-methylxanthine (IBM XN), insulin, dexamethasone, and indomethacin; Common inducers for osteogenic differentiation include dexamethasone or vitamin D _ 3, ascorbic acid, and sodium 尾 -glycerophosphate; Basic fibroblast growth factor, epidermal growth factor and vitamin B27 can be combined to induce neural differentiation of adipose stem cells. 5-azacytidine is the commonly used inducer of cardiomyocyte differentiation. The combination of vascular endothelial growth factor and basic fibroblast growth factor can induce adipose stem cells to differentiate into vascular endothelial cells, with the rapid development of molecular biology and cell biology. The differentiation of adipose stem cells will also be more in-depth, on the basis of the current observation of adipose stem cell induced differentiation. The study of its intrinsic molecular mechanism and genes and proteins regulating the plasticity of adipose stem cells should be strengthened.
【作者单位】：中国医科大学基础医学院病原生物学教研室;
【分类号】：R329.2
【正文快照】： 文章亮点:1此问题的已知信息:脂肪干细胞能诱导分化为同样中胚层来源的成骨细胞、脂肪细胞、软骨细胞,还可以跨胚层分化为内胚层来源的肝细胞、胰岛β样细胞,外胚层来源的神经细胞、心肌细胞、表皮细胞等。2文章增加的新信息:详细阐述了脂肪干细胞向脂肪细胞、骨细胞、软骨细

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