人胚胎来源多潜能干细胞向脂肪和神经细胞分化的研究

发布时间：2018-01-07 16:17

本文关键词：人胚胎来源多潜能干细胞向脂肪和神经细胞分化的研究　出处：《吉林大学》2010年硕士论文　论文类型：学位论文

【摘要】： 人胚胎肝组织来源的多潜能干细胞是从胎儿肝组织中分离的一类干细胞,与较之前报道的成体干细胞(如骨髓间充质干细胞)有很多相似的特性,具有多分化潜能并能够形成新的组织和器官,具有重要的医学治疗作用。本研究对人胚胎肝组织来源的多潜能干细胞进行分离和扩增,对其生物学特性进行了研究,探讨了人胚胎来源多潜能干细胞向脂肪细胞和神经细胞方向诱导分化的潜能。采用胶原酶消化法分离人胚胎肝组织来源多潜能干细胞,经过体外培养和扩增,对人胚胎肝组织来源多潜能干细胞的生长经时变化和表面标志CD105、CD166、CD73和HLA-DR进行检测。应用成脂诱导培养液(地塞米松、3-异丁基-1-甲基黄嘌呤、吲哚美辛、胰岛素)诱导其向脂肪细胞分化,使用化学诱导法诱导其向神经细胞方向分化,然后进行鉴定。结果表明,人胚胎肝组织来源多潜能干细胞的生物学特性与骨髓间充质干细胞类似,免疫荧光化学染色及RT-PCR结果说明人胚胎肝组织来源多潜能干细胞经诱导可分化成为脂肪细胞及神经细胞。本研究结果提示,人胚胎来源多潜能干细胞具有分化成脂细胞和神经细胞的潜能,有望应用于组织工程及细胞治疗的种子细胞。
[Abstract]:Pluripotent human embryonic liver tissue derived stem cells are isolated from liver tissue of the fetus, and compared with the previously reported adult stem cells (such as bone marrow mesenchymal stem cells) have many similar characteristics, with multiple differentiation potential and can form new tissues and organs, with medical treatment an important role.
The study of pluripotent human embryonic liver tissue derived stem cells were isolated and amplified, its biological characteristics were studied, discussed the human embryonic pluripotent stem cells differentiate into fat cells and nerve cells. The direction of the potential separation of human embryonic pluripotent stem cells by collagenase digestion, and after in vitro culture of human embryonic liver tissue CD166 pluripotent stem cell growth by the change and the surface markers CD105, CD73 and HLA-DR were detected. Application of adipogenic induction medium (dexamethasone, 3- -1- isobutyl methylxanthine, indomethacin, insulin) to induce adipocyte differentiation, induction method to induce nerve cell differentiation using chemical, and then were identified. The results showed that human embryonic pluripotent stem cells and biological characteristics of bone marrow mesenchymal stem cells is similar to that of free Immunofluorescence staining and RT-PCR results indicated that human embryonic pluripotent stem cells can be induced into fat cells and nerve cells. The results of this study suggest that human embryonic pluripotent stem cells can differentiate into fat cells and nerve cell potential, is expected to be used as seed cells for tissue engineering and cell therapy.

【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R329

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