1-磷酸鞘氨醇对心肌细胞离子通道作用的实验研究

发布时间：2018-01-10 12:31

本文关键词：1-磷酸鞘氨醇对心肌细胞离子通道作用的实验研究　出处：《吉林大学》2008年硕士论文　论文类型：学位论文

【摘要】： 目的:通过全细胞膜片钳技术、单通道膜片钳技术、台盼蓝染色技术观察S1P对豚鼠心室肌细胞L型钙电流(IL-Ca)、延迟整流钾电流(IK)及氧化损伤后心肌细胞凋亡的影响,进一步探讨其心肌保护作用的机理。方法:应用全细胞膜片钳技术,观察不同剂量S1P对豚鼠心室肌细胞L型钙电流(IL-Ca)电流幅值的影响。应用单通道膜片钳技术,观察S1P对豚鼠心室肌细胞延迟整流钾电流(IK)开放时间、关闭时间、开放概率的影响。通过台盼蓝染色技术观察不同剂量S1P对H2O2氧化损伤后心肌细胞凋亡的影响。结果:不同剂量S1P对豚鼠心室肌细胞L型钙电流(IL-Ca)均无作用;S1P可使豚鼠心室肌细胞延迟整流钾电流(IK)的开放时间缩短,关闭时间延长,开放概率降低,与对照组相比较,P0.05,差异显著;S1P剂量依赖性的抑制氧化损伤后的心肌细胞凋亡,大、中剂量组与模型组比较,P0.001,差异显著。结论: S1P可以使豚鼠心室肌细胞延迟整流钾电流(IK)的开放时间缩短,关闭时间延长,开放概率降低,抑制整个细胞延迟整流钾电流(IK)外流,S1P可以抑制氧化损伤后的心肌细胞凋亡,上述结果均说明是S1P对急性心梗后缺血缺氧的心肌有一定的保护作用。
[Abstract]:Objective: to observe the effect of S1P on L-type calcium current (L-type calcium current) in guinea pig ventricular myocytes by whole-cell patch clamp technique, single-channel patch clamp technique and trypan blue staining. The effects of delayed rectifier potassium current (IKK) and oxidative injury on cardiomyocyte apoptosis and the mechanism of myocardial protection were discussed. Methods: the effects of different doses of S1P on the amplitude of L-type calcium current and IL-Ca1 in guinea pig ventricular myocytes were observed by whole-cell patch clamp technique. To observe the opening time and closing time of S1P to delayed rectifier potassium current in guinea pig ventricular myocytes. Effects of different doses of S _ 1P on apoptosis of cardiomyocytes after oxidative injury of H _ 2O _ 2 were observed by trypan blue staining. Results: different doses of S1P had no effect on L-type calcium current and IL-Ca1 in guinea pig ventricular myocytes. S1P could shorten the opening time, prolong the closing time and decrease the open probability of delayed rectifier potassium current in guinea pig ventricular myocytes, which was significantly different from that of control group (P 0.05). S1P dose-dependently inhibited the apoptosis of cardiomyocytes after oxidative injury, and the difference was significant between the middle dose group and the model group (P 0.001). Conclusion: S1P can shorten the opening time, prolong the closing time and decrease the open probability of delayed rectifier potassium current in guinea pig ventricular myocytes. Inhibiting the whole cell delayed rectifier potassium current (IKK) efflux (S1P) can inhibit the apoptosis of cardiomyocytes after oxidative injury. These results suggest that S1P has a protective effect on myocardial ischemia and hypoxia after acute myocardial infarction.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R331.3

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