龙血素A对白色假丝酵母菌毒力因子ALS3、SAP4作用的体外研究

发布时间：2018-01-12 09:08

本文关键词：龙血素A对白色假丝酵母菌毒力因子ALS3、SAP4作用的体外研究　出处：《中国微生态学杂志》2015年06期 　论文类型：期刊论文

【摘要】：目的研究龙血素A对白色假丝酵母菌生物膜的抑菌作用,并初步研究龙血素A是否通过影响或干扰白色假丝酵母菌毒力因子的转录和表达实现的。方法建立白色假丝酵母菌生物膜体外模型,MTT法计算龙血素A对白色假丝酵母菌生物膜的抑制率;激光共聚焦观察龙血素A对不同时间白色假丝酵母菌生物膜的抑菌效果;RT-PCR技术检测药物作用下白色假丝酵母菌毒力因子表达水平的变化。结果随着药物浓度的增加,龙血素A对白色假丝酵母菌生物膜的抑菌作用增强。激光共聚焦观察显示龙血素A使白色假丝酵母菌生物膜内活菌死菌比例下降。RT-PCR结果表明龙血素A抑制了毒力因ALS3、SAP4的表达。结论龙血素A对白色假丝酵母菌生物膜有抑制作用,并在毒力因子ALS3、SAP4的表达过程中起到明显的抑制作用。
[Abstract]:Objective to study the bacteriostatic effect of dragon blood A on Candida albicans biofilm. And the preliminary study on whether dragon blood A by affecting or interfering with the virulence factor transcription and expression of Candida albicans. Methods the model of Candida albicans biofilm in vitro was established. The inhibitory rate of dragonin A on Candida albicans biofilm was calculated by MTT method. Laser confocal focusing was used to observe the bacteriostatic effect of dragon blood A on the biofilm of Candida albicans at different time. The expression of virulence factor in Candida albicans was detected by RT-PCR. The bacteriostatic effect of dragon blood A on the biofilm of Candida albicans was enhanced. Laser confocal observation showed that the proportion of live bacteria in the biofilm of Candida albicans was decreased by dragon blood A. RT-PCR results showed that dragon blood in the biofilm of Candida albicans was reduced. A inhibited virulence factor ALS3. Conclusion Dragonin A can inhibit the biofilm of Candida albicans and play a significant role in the expression of virulence factor ALS3 / SAP4.
【作者单位】：佳木斯大学附属第二医院牙体牙髓病二科;
【分类号】：R379.4
【正文快照】： 近年来研究发现,根管生物膜的存在是导致牙髓感染性疾病经久不愈及根管治疗失败的主要原因,这与根管生物膜的结构特殊、组成多样化,耐药性及具有抵抗宿主的免疫反应能力有密切联系。白色假丝酵母菌是人体正常菌群之一,广泛存在于口腔、呼吸道、肠道等部位[1],同时也是一种常见

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