肺炎克雷伯杆菌噬菌体的分离鉴定和生物学特性的研究

发布时间：2018-01-16 16:17

本文关键词：肺炎克雷伯杆菌噬菌体的分离鉴定和生物学特性的研究　出处：《吉林大学》2009年硕士论文　论文类型：学位论文

【摘要】： 抗生素一直被认为是治疗细菌感染性疾病最有力的武器,但随着致病菌对大多数抗生物产生抗性的出现,细菌的耐药性已成为现代医学中的一个非常严峻的问题。人们担忧人类正重新进入“前抗生素”时代。噬菌体作为一种新兴的治疗方法,已经越来越受到人们的关注。噬菌体对细菌具有高度专一性,当噬菌体侵染细菌时,可在细菌中繁殖并杀死细菌,而对动植物没有毒性。本研究以肺炎克雷伯杆菌噬菌体为主要内容,从居民区污水中分离出二株肺炎克雷伯杆菌噬菌体,分别命名为K1BP108和K1BP109。同时对其生物学性状进行了初步的研究。结果表明,利用噬斑法能获得比较纯的噬菌体,电镜照片显示为立体对称型,未见明显的尾部;所分离到的肺炎克雷伯杆菌噬菌体的基因组为双链DNA,大小约25kb左右;在pH6.5~10的范围内噬菌体保持较好的裂解活性;在温度60℃条件下,噬菌体K1BP108稳定,生长曲线显示噬菌体感染宿主菌的潜伏时间为31分钟,爆发时间为47分钟;在紫外线照射下18分钟噬菌体全部被灭活。肺炎克雷伯杆菌是革兰氏阴性菌,是医院内感染重要的病原菌之一,其耐药性也呈逐年增加趋势,开发和研究针对肺炎克雷伯杆菌的噬菌体制剂,无疑是拓展抵御细菌感染的新领域。本研究探讨了肺炎克雷伯杆菌噬菌体的生物学特性,为进一步研制和开发针对肺炎克雷伯杆菌感染,特别是针对耐药和多重耐药菌株的噬菌体打下基础。
[Abstract]:Antibiotic treatment of bacterial infections has been considered to be the most powerful weapon of disease, but with the most anti biological resistance emergence of pathogenic bacteria, bacterial resistance has become a serious problem in modern medicine. People worry about "era anti humanity is re entering the" students. As a new phage the treatment method has attracted more and more attention. The phage has a high degree of specificity of bacteria, when phage infection bacteria, in bacteria breeding and kill bacteria, and no toxicity to plants and animals.
In this study, Klebsiella pneumoniae phage as the main content, two strains of Klebsiella pneumoniae phages from residential areas in the sewage, which were named as K1BP108 and K1BP109. also conducted a preliminary study on its biological characters. The results showed that by plaque assay can obtain relatively pure phage as electron micrograph the three-dimensional symmetrical type, there is no obvious tail; isolated Klebsiella pneumoniae phage genome into double stranded DNA, the size is about 25KB; in the range of pH6.5~10 to maintain good phage lytic activity; at a temperature of 60 DEG C, phage K1BP108 stable, growth curve showed that the latent time of host bacteria phage infection for 31 minutes, the onset time is 47 minutes; 18 minutes under UV irradiation were all phage inactivated.
Klebsiella pneumoniae is a gram negative bacterium, is one of the important pathogens of nosocomial infection and its drug resistance has increased year by year, the research and development of the phage preparations of Klebsiella pneumoniae, is expanding new areas against bacterial infection. This study explores the biological characteristics of Klebsiella pneumoniae phage, for further research and development for Klebsiella pneumoniae infection, especially for drug resistance and multiple drug resistant strains of phage to lay the foundation.

【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R516;R378

【引证文献】