利用月经血建立的经血源性基质干细胞系

发布时间：2018-01-20 00:54

本文关键词： 成体干细胞月经细胞系组织工程干细胞培养经血源性基质干细胞表型鉴定细胞建系细胞治疗　出处：《中国组织工程研究》2015年50期 　论文类型：期刊论文

【摘要】：背景:经血源性基质干细胞被证实是一种新型的成体干细胞,目前国内关于其系统的建系方法报道极少。目的:建立经血源性基质干细胞系,并鉴定其表型和多能性。方法:收集健康成年女性的月经血,通过密度梯度离心法分离出经血源性基质干细胞进行培养,观察细胞的形态及增殖特性;采用CCK-8法检测各株细胞的增殖能力,定期收集细胞进行核型分析,采用流式细胞术检测细胞表面抗原CD34,CD38,CD44,CD45,CD73,CD90,CD105,Oct-4的表达,对细胞进行体外成脂和成骨诱导分化并鉴定。结果与结论:成功从6名健康志愿者的经血中分离获得4株经血源性基质干细胞系。细胞呈现典型的梭状结构,具有46,XX核型,能在体外稳定迅速增殖,传代后大约48 h进入对数生长期,表达CD44,CD73,CD90,CD105及Oct-4,不表达CD38,CD34,CD45;成脂诱导22 d后油红O染色阳性,成骨诱导2周后茜素红染色阳性。实验表明经血源性基质干细胞具备扩增迅速、核型稳定、多潜能性等优点,通过建立经血源性基质干细胞系,为进一步的临床治疗应用以及科学研究奠定了基础。
[Abstract]:Background: menstrual blood derived stromal stem cells have been proved to be a new type of adult stem cells. Methods: the menstrual blood of healthy adult women was collected and cultured by density gradient centrifugation. The morphology and proliferation of the cells were observed. The proliferative ability of each cell line was detected by CCK-8 assay, and the cell karyotype was collected regularly. Flow cytometry was used to detect the cell surface antigen CD34, CD38, CD44 and CD45. Expression of CD73, CD90, CD105, Oct-4. Results and conclusion: four blood derived stromal cell lines were successfully isolated from the blood of 6 healthy volunteers. The cells showed a typical fusiform structure. It can proliferate stably and rapidly in vitro, enter logarithmic growth phase about 48 hours after passage, and express CD44T CD73, CD90, CD105 and Oct-4. CD38, CD34, CD45; Oil red O staining was positive after adipogenic induction 22 days later, and alizarin red staining was positive 2 weeks after osteogenesis induction. The results showed that hematogenous stromal stem cells had the advantages of rapid expansion, stable karyotype and multipotential. The establishment of meridian blood derived stromal stem cell lines lays a foundation for further clinical application and scientific research.
【作者单位】：中国医科大学附属盛京医院;
【基金】：辽宁省科学技术计划项目(2012225014)~~
【分类号】：R329.2
【正文快照】： 0引言Introduction干细胞是增殖发育形成人体各种组织细胞的最初来源。1961年,Till等[1]首次提出了干细胞的基本特性,即具有自我更新,无限增殖以及多向分化的潜能。干细胞根据发育阶段可以分为胚胎干细胞和成体干细胞两类。胚胎干细胞是一种能够向所有特定器官细胞类型分化的

【相似文献】