白消安联合环磷酰胺诱导小鼠急性移植物抗宿主病模型的建立

发布时间：2018-01-20 09:18

本文关键词： 白消安环磷酰胺骨髓移植急性移植物抗宿主病医用实验动物学　出处：《细胞与分子免疫学杂志》2015年12期 　论文类型：期刊论文

【摘要】：目的以白消安(BS)联合环磷酰胺(CP)作为移植前预处理方案建立稳定的小鼠急性移植物抗宿主病(a GVHD)模型。方法雄性BALB/c(H-2kd)为受体鼠,雌性C57BL/6(H-2kb)为供鼠,受体鼠接受100 mg/kg BS和200 mg/kg CP预处理后,输入2×107个供鼠的骨髓细胞或2×107个骨髓细胞联合2×107个脾细胞。记录移植后小鼠一般情况,观察a GVHD临床表现,HE染色后检查组织病变情况,流式细胞术检测脾脏、骨髓中细胞嵌合度。结果移植骨髓联合脾细胞组小鼠,移植后7 d开始出现典型a GVHD临床表现,肝、脾、肠、皮肤、肺等组织出现GVHD相关病理形态学改变,并达到完全供者嵌合,中位生存期为10 d,30 d内全部死亡;单移植骨髓细胞组小鼠无a GVHD表现,生存期大于45 d。结论成功建立了稳定可靠的a GVHD小鼠模型,可用于a GVHD的发病机制、防治效果的实验性研究。
[Abstract]:Objective to establish a stable mouse model of acute graft-versus-host disease (AGV HDD) with BSand cyclophosphamide (CPP) as a preconditioning regimen before transplantation. H-2kd) is a receptor mouse. The recipient mice were pretreated with 100 mg/kg BS and 200 mg/kg CP. Bone marrow cells of 2 脳 107 donor mice or 2 脳 107 bone marrow cells combined with 2 脳 107 spleen cells were injected. The general situation of mice after transplantation was recorded and the clinical manifestations of a GVHD were observed. After HE staining, the pathological changes of tissues were examined, and the chimerism of spleen and bone marrow cells were detected by flow cytometry. On the 7th day after transplantation, typical clinical manifestations of a GVHD began to appear, liver, spleen, intestine, skin, lung and other tissues showed pathomorphological changes related to GVHD, and achieved complete donor chimerism. The median survival time was 10 days and all died within 30 days. The survival time was longer than 45 days. Conclusion the stable and reliable model of a GVHD mice was established successfully, which can be used for the pathogenesis of a GVHD. Experimental study on the effect of prevention and cure.
【作者单位】：南方医科大学珠江医院血液内科;中山大学附属中山医院血液内科;
【基金】：广东省科技计划项目[粤科社字(2011)106号] 中山市科技计划重大专项(20113A002);中山市科技计划(20122A111)
【分类号】：R-332;R457.7
【正文快照】： 异基因造血干细胞移植(allogeneic hematopoieticstem cell transplantation,allo-HSCT)是目前许多血液系统疾病、恶性肿瘤及遗传性疾病的有效治疗方法。然而供者源免疫活性T细胞在重建受者免疫系统发挥移植物抗白血病或淋巴瘤效应(graft-versus-leukemia/lymphoma,GVL)的同时

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