牛蒡子苷元对免疫性血小板减少模型的药效及机理

发布时间：2018-01-23 04:09

本文关键词： 牛蒡子苷元免疫性血小板减少动物模型调节性T细胞　出处：《沈阳药科大学学报》2015年04期 　论文类型：期刊论文

【摘要】：目的考察牛蒡子苷元对免疫性血小板减少疾病(immune thrombocytopenic purpura,ITP)动物模型的药效学,并初步研究其作用机制。方法采用SD大鼠腹腔注射兔抗大鼠血小板(platelet,PLT)抗体血清制备被动型ITP动物模型,Balb/c小鼠腹腔注射大鼠血小板抗原制备小鼠交叉免疫反应型ITP模型,SD大鼠皮下注射环磷酰胺制备大鼠药物诱导型血小板减少模型,通过血细胞计数仪检测以上3种动物模型全血PLT数量,并研究牛蒡子苷元对ITP模型的药效作用;正常Balb/c小鼠给予牛蒡子苷元1.0 mg·kg-1·d-1后,利用流式技术检测脾脏淋巴细胞Treg细胞比率,研究牛蒡子苷元药理作用机制。结果成功构建3种ITP模型,牛蒡子苷元能显著性提高被动型ITP模型与交叉免疫反应型ITP模型的PLT数量,与模型组相比有显著性差异(P0.05);对环磷酰胺造成的模型作用不明显(P0.05);牛蒡子苷元能显著性提高正常小鼠脾脏淋巴细胞Treg细胞比率(P0.05)。结论牛蒡子苷元对免疫性血小板减少动物模型取得较好的治疗效果,这可能与其升高Treg细胞比率有关。
[Abstract]:Objective to investigate the pharmacodynamics of arctiin on immune thrombocytopenic purpura (thrombocytopenic) model of immune thrombocytopenia. Methods the passive ITP animal model was established by intraperitoneal injection of rabbit anti-platelet platelet (PLT) antibody serum from SD rats. Balb/c mice were injected intraperitoneally with rat platelet antigen to establish cross-immunoreactive ITP model and SD rats were subcutaneously injected with cyclophosphamide to prepare drug-induced thrombocytopenia model. The whole blood PLT of the above three animal models was detected by blood cell counter, and the effect of arctiin on ITP model was studied. The ratio of Treg cells in spleen lymphocytes was detected by flow cytometry after normal Balb/c mice were given lappadin 1.0 mg 路kg-1 路d-1. The pharmacological mechanism of arctiin was studied. Results three ITP models were successfully constructed. Arctiin could significantly increase the number of PLT in passive ITP model and cross-immunoreactive ITP model. There was significant difference between the model group and the model group (P 0.05). The effect on the model caused by cyclophosphamide was not obvious (P 0.05). Arctigenin can significantly increase the ratio of Treg cells in spleen lymphocytes of normal mice (P 0.05). Conclusion Arctigenin has a good therapeutic effect on immune thrombocytopenia animal model. This may be related to its increased Treg cell ratio.
【作者单位】：沈阳药科大学生命科学与生物制药学院;鲁南制药集团药理中心;
【分类号】：R285.5;R-332
【正文快照】： 免疫性血小板减少(immune thrombocytopenicpurpura,ITP)是常见的以凝血障碍为特征的自身免疫性疾病,临床治疗以口服糖皮质激素与静脉注射免疫球蛋白为主要方法。ITP为慢性病,长期口服激素治疗副作用大,注射免疫球蛋白费用高[1-2],因此从祖国传统医学寻找治疗药物具有重要意义

【参考文献】