非人灵长类动物急性肝功能衰竭模型的建立

发布时间：2018-01-25 14:24

本文关键词： 急性肝功能衰竭食蟹猴 D-氨基半乳糖胺动物模型　出处：《世界华人消化杂志》2010年19期 　论文类型：期刊论文

【摘要】：目的:构建一种稳定的非人灵长类大动物急性肝衰竭模型.方法:将15只食蟹猴随机分成3组,经颈外静脉分别注射0.45、0.3和0.15g/kgD-氨基半乳糖胺,构建食蟹猴肝衰竭动物模型.动态检测给药前(0h)、给药后12、24、36、48、60、72、96h生命体征、颅内压(ICP)及血清丙氨酸转氨酶(ALT)、凝血酶原时间(PT)、总胆红素(TBIL)、血氨(NH3)的变化.观察动物临床表现及病程进展情况,记录动物生存时间,动物死亡后行组织病理学检查.结果:0.45g/kg和0.3g/kg组动物全部死亡,生存时间分别为56.1h±8.1h和109.8h±11.2h,0.15g/kg实验猴除1只98h死亡外其余全部存活.0.45g/kg和0.3g/kg组动物各时点的ALT、TBIL、PT、NH3及ICP均明显高于基线值(0h),差异有统计学意义(P0.05),0.15g/kg实验猴各指标在60h后渐恢复正常.死亡动物组织病理检查见肝细胞坏死,肝内炎性细胞浸润.结论:以0.3g/kg的D-氨基半乳糖胺诱发食蟹猴急性肝功能衰竭动物模型,符合急性肝功能衰竭的临床表现、病理变化,可用于急性肝功能衰竭的动物实验研究.
[Abstract]:Objective: to establish a stable animal model of acute liver failure in non-human primate. Methods: 15 monkey were randomly divided into 3 groups and injected into external jugular vein 0.45 respectively. The animal model of liver failure in crab monkey was established by 0.3 and 0.15 g / kg D- galactosamine. 96 h vital signs, ICP (ICP), serum alanine aminotransferase (alt), prothrombin time (PTT), total bilirubin (TBIL). The changes of NH _ 3 in blood were observed, the clinical manifestation and progression of the disease were observed, and the survival time of the animals was recorded. Histopathological examination was performed after the death of the animals. Results all the animals in the 0.45g / kg and 0.3g / kg groups died. The survival time was 56.1 h 卤8.1 h and 109.8 h 卤11.2 h respectively. All the experimental monkeys of 0.15 g / kg survived with the exception of one animal died for 98 hours. All the animals in the 0.45 g / kg and 0.3 g / kg groups were treated with alt TBILPPT at different time points. NH3 and ICP were significantly higher than baseline value (P 0.05). The indexes of 0.15 g / kg monkey gradually returned to normal after 60 hours, and liver cell necrosis was found in pathological examination of dead animals. Conclusion: the animal model of acute hepatic failure induced by 0.3 g / kg D-galactosamine is consistent with the clinical manifestations and pathological changes of acute hepatic failure. It can be used in the animal experimental study of acute liver failure.
【作者单位】：南方医科大学附属珠江医院肝胆二科;广东省昆虫研究所华南灵长类研究开发中心;
【基金】：国家高技术研究发展计划基金资助项目(863计划),No.2006AA02A141~~
【分类号】：R575.3
【正文快照】： 0引言急性肝功能衰竭(acute liver failure,ALF)病情复杂、影响因素多、缺乏对照而难以确切判断疗效.临床上为研究ALF的发病机制和治疗多以啮齿类、犬类动物建立ALF模型,目前国内外尚未见以非人灵长类动物建立的ALF模型的报道.1材料和方法1.1材料食蟹猴(Macaca Faiscularis)1

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