慢性HBV感染者外周血单个核细胞共刺激分子和FOXP3 mRNA的表达

发布时间：2018-01-26 12:27

本文关键词： 共刺激分子 B7-2 PD-L1 CD4~+CD25~+调节性T细胞乙型肝炎病毒免疫耐受　出处：《暨南大学》2009年硕士论文　论文类型：学位论文

【摘要】： 目的探讨外周血单个核细胞共刺激分子B7-2和PD-L1及CD4~+CD25~+调节性T细胞FOXP3mRNA表达在慢性乙型肝炎患者免疫耐受中的作用。方法以慢性HBV感染免疫清除期患者30例、免疫耐受期患者10例和健康对照10例为研究对象,用RT-PCR方法研究外周血单个核细胞共刺激分子B7-2和PD-L1及FOXP3mRNA的表达水平。结果免疫清除期、免疫耐受期患者和健康对照组B7-2的表达水平分别为0.826±0.055、0.880±0.036和0.942±0.029;免疫清除期和免疫耐受期患者B7-2的表达水平均显著低于健康对照组(P分别为0.000和0.002);免疫清除期患者B7-2的表达水平低于免疫耐受期患者(P=0.004)。免疫清除期、免疫耐受期患者PD-L1的表达分别为0.763±0.090、0.741±0.032,均高于健康对照组的0.663±0.040(P分别为0.000和0.000);免疫清除期患者PD-L1的表达水平高于免疫耐受期患者,但两者差异没有统计学意义(P=0.564)。免疫清除期、免疫耐受期PD-L1/B7-2比值分别为0.926±0.102、0.842±0.049,均显著高于健康对照组的0.704±0.042(P分别为0.000和0.000);免疫清除期患者PD-L1/B7-2比值高于免疫耐受期患者,统计学处理有显著性差异(P=0.005)。免疫清除期、免疫耐受期和健康对照FOXP3的表达分别为0.864±0.085、0.621±0.041和0.603±0.037;免疫清除期患者FOXP3的表达显著高于免疫耐受期患者(P=0.000)和健康对照组(P=0.000),免疫耐受期患者FOXP3的表达稍高于健康对照组,但差异尚无统计学意义(P=0.675)。免疫清除期PD-L1/B7-2比值与FOXP3表达呈显著正相关(r=0.491,P=0.006)。结论在慢性HBV感染中,正、负共刺激分子的变化可能是机体对免疫反应保护性调节的结果,最终可能使机体对HBV的免疫耐受加深。正负共刺激分子和FOXP3 mRNA的表达有相关性;共刺激分子和CD4~+CD25~+调节性T细胞可能共同参与调节机体对HBV感染的免疫耐受。
[Abstract]:Purpose Study on the costimulatory molecules B7-2, PD-L1 and CD4 ~ CD25 ~ in peripheral blood mononuclear cells. The role of regulatory T cell FOXP3mRNA expression in immune tolerance in patients with chronic hepatitis B. Method Thirty patients with chronic HBV infection, 10 patients with immune tolerance and 10 healthy controls were studied. The expression levels of B7-2, PD-L1 and FOXP3mRNA in peripheral blood mononuclear cells (PBMC) were studied by RT-PCR. Results The expression levels of B7-2 in the immune clearance phase, the immune tolerance stage patients and the healthy control group were 0.826 卤0.0555 卤0.880 卤0.036 and 0.942 卤0.029, respectively. The expression of B7-2 in immune clearance phase and immune tolerance stage was significantly lower than that in healthy control group (P = 0.000 and 0.002), respectively. The expression level of B7-2 in patients with immune clearance was lower than that in patients with immune tolerance. The expression of PD-L1 in patients with immune tolerance was 0.763 卤0.090, 0.741 卤0.032, respectively. It was 0.000 and 0.000m respectively higher than that of the healthy control group (0.663 卤0.040). The expression of PD-L1 was higher in patients with immune clearance than in patients with immune tolerance, but there was no significant difference between the two groups. The ratio of PD-L1/B7-2 in immune tolerance phase was 0.926 卤0.102 卤0.842 卤0.049, respectively. All of them were significantly higher than those of the healthy control group (0.704 卤0.042) P = 0.000 and 0.000, respectively. The ratio of PD-L1/B7-2 in patients with immune clearance was higher than that in patients with immune tolerance. The expression of FOXP3 was 0.864 卤0.085, 0.621 卤0.041 and 0.603 卤0.037 in immune tolerance period and healthy control, respectively. The expression of FOXP3 in patients with immune clearance was significantly higher than that in patients with immune tolerance (0.000) and healthy controls (0.000). The expression of FOXP3 in patients with immune tolerance was slightly higher than that in healthy controls. However, there was no statistical significance between the PD-L1/B7-2 ratio and the expression of FOXP3. There was a significant positive correlation between the PD-L1/B7-2 ratio and the expression of FOXP3. Conclusion In chronic HBV infection, the changes of positive and negative costimulatory molecules may be the result of protective regulation of immune response. The positive and negative costimulatory molecules were correlated with the expression of FOXP3 mRNA. Costimulatory molecules and CD4 ~ CD25 ~ regulatory T cells may be involved in the regulation of immune tolerance to HBV infection.
【学位授予单位】：暨南大学
【学位级别】：硕士
【学位授予年份】：2009
【分类号】：R392

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