输注供体未成熟树突状细胞对大鼠皮肤移植的影响

发布时间：2018-01-28 18:30

本文关键词： 未成熟树突状细胞免疫耐受皮肤移植大鼠　出处：《中国医科大学》2008年硕士论文　论文类型：学位论文

【摘要】： 目的建立稳定的大鼠皮肤移植排斥反应模型,探讨输注供体未成熟树突状细胞减轻排斥反应和诱导免疫耐受的作用。方法通过建立大鼠自体皮肤移植模型,熟练掌握大鼠皮肤移植方法。继而用改良的方法扩增未成熟树突状细胞:rhG-CSF,5μg/kg,皮下注射,连续7天后,从骨髓分离、提取树突状细胞,体外培养3d。实验分组:自体移植组、对照1组SD→Wistar、对照2组Wistar→Wistar、和imDC实验组SD→Wistar,配对组合皮肤移植。于皮肤移植术中经阴茎背静脉输注供体未成熟树突状细胞5×10~6个,动态观察术后移植皮肤的存活时间,不同组别移植物病理改变,探讨供体imD减轻大鼠皮肤移植免疫排斥反应的作用。结果 1、全部自体移植皮片至观察终点(＞2月)仍然存活,移植皮片与周围组织愈合良好,无缺损或破溃,色泽与周围组织无明显区别。移植物表皮及附属毛发、毛囊生长良好。术后14d镜下观察移植物病理切片可见:各组织层次清晰完整,皮肤附属结构完好,可以看到完整的毛囊结构,细胞结构完好,无明显的炎性细胞浸润。2.对照1组,移植皮片在14天内全部被排斥掉,排斥反应发生早,且严重。移植物的平均存活时间仅为11.8±1.6d,最短者于术后第9d完全排斥。3.对照2组:Wistar→Wistar组,虽然移植物最终全部被排斥,但排斥反应发生晚且较轻,平均生存期为23.1±2.4d。移植物最长存活27d,最短存活19d。术后14d镜下移植物病理切片表现:移植物组织结构不完整,附属结构被破坏,未见正常毛囊结构,可见部分坏死灶,有大量炎细胞浸润。于高倍镜下可见组织细胞核溶解、碎裂。4.实验组,全部移植物在25d内被排斥,存活时间最短14d,平均存活时间为19.3±3.4d,最短14d。术后14d镜下移植物病理切片表现:移植物组织结构遭破坏,可见坏死灶,有大量炎细胞浸润。于高倍镜下可见组织细胞核溶解、碎裂。结论使用供体imDC预处理受体鼠可以显著延长移植物的存活时间,减轻排斥反应。
[Abstract]:Purpose To establish a stable model of skin allograft rejection in rats and to explore the role of donor immature dendritic cells in reducing rejection and inducing immune tolerance. Method The rat skin autograft model was established to master the method of rat skin transplantation, and then the immature dendritic cells were amplified by 5 渭 g / kg and injected subcutaneously with a modified method. After 7 days, dendritic cells were isolated from bone marrow and cultured for 3 days in vitro. Experimental group: autologous transplantation group, control group 1 SD 鈫扺istar, control group 2 Wistar. 鈫扺istar, and imDC experimental group SD. 鈫扵he donor immature dendritic cells (5 脳 10 ~ 6) were injected through the dorsal penile vein to observe the survival time of the skin graft. Objective: to investigate the role of donor imD in alleviating the immune rejection of skin transplantation in rats. Results 1. All the autografted skin grafts survived until the observed end point (> February). The skin grafts healed well with the surrounding tissues, and there was no defect or rupture. There was no significant difference in color and color between the grafts and surrounding tissues. The skin follicles grew well with the grafts epidermis and hair. The histological sections of the grafts were clear and complete under the microscope 14 days after operation. It can be seen that the complete hair follicle structure, cell structure intact, no obvious inflammatory cell infiltration. 2. In the control group 1, all the skin grafts were rejected within 14 days, the rejection occurred early. The mean survival time of the graft was only 11.8 卤1.6 days, and the shortest graft was completely rejected on the 9th day after operation. 鈫扞n the Wistar group, although all the grafts were eventually rejected, the rejection occurred later and lighter, with an average survival time of 23.1 卤2.4 days. The graft survived for the longest 27 days. The shortest survival time was 19 days. The histologic features of the grafts were incomplete, the accessory structure was destroyed, the normal hair follicle structure was not seen, and some necrotic foci were observed at the 14th day after operation. A large number of inflammatory cells infiltrated. Under the high power microscope, the tissue nucleus was dissolved, fragmentation. 4. In the experimental group, all the grafts were rejected within 25 days, the shortest survival time was 14 days. The mean survival time was 19.3 卤3.4 days and the shortest was 14 days. A large number of inflammatory cells infiltrated. Under the high power microscope, the nucleus of the tissue was dissolved and broken. Conclusion Pretreatment with donor imDC could significantly prolong the survival time of the grafts and alleviate the rejection.
【学位授予单位】：中国医科大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R392

【参考文献】