低钠盐对胰岛素信号通路基因网络状态的影响

发布时间：2018-01-30 07:50

本文关键词： 胰岛素抵抗低钠盐胰岛素信号通路表达谱芯片局部网络　出处：《天津科技大学》2008年硕士论文　论文类型：学位论文

【摘要】： 随着人类基因组测序工作的完成,基因与饮食的关系已经成为营养学研究的新热点。在流行病学研究中,发现高血压与肥胖均属于多基因疾病,且都存在胰岛素抵抗,即胰岛素信号通路传导异常。限盐作为有效的非药物手段,在控制血压、肥胖及缓减胰岛素抵抗方面有着积极作用。基于以上的研究,本论文应用基因芯片技术,从分子水平,揭示低钠盐饮食对胰岛素信号通路相关基因表达情况的影响,并建立基因间相互关联的局部网络。论文中所用的低钠盐由川崎食品有限公司提供,其NaCl含量为50%左右。在监督下,用这种盐代替普通食盐,针对正常人群、高血压人群、以及高血压并发肥胖人群,共9人,进行12周的实验。采集受试者食用前后血样,进行芯片实验。本论文通过生物信息统计,确定血液中表达的胰岛素信号通路相关基因共114个。针对高血压人群与正常人群,应用博奥生物有限公司的22K人类全基因组芯片。主要的变化基因Akt2、Akt3、GSK3、GCK、HK2、以及SOS参与了相关通路：PI-3K/Akt Signaling Pathway, Focal Adhesion, T Cell Receptor Signaling Pathway, JAK-STAT Signaling Pathway。针对高血压并发肥胖患者人群,应用康成生物工程公司针对性更强的芯片,仅对114个胰岛素信号通路相关基因进行检测。由表达明显变化的基因建立基因局部网络,主要涉及到的通路有：PI-3K/Akt Signaling Pathway, Focal Adhesion,B Cell Reception Signaling Pathway, long-term depression。通过对这些基因局域网络的分析,发现这些基因表达的变化对胰岛素信号相关通路的传导有一定改善作用,调控细胞的活性,改善葡萄糖的摄取和运输,在一定程度上能缓减胰岛素抵抗。希望本论文能为限盐研究提供新的思路和线索。
[Abstract]:With the completion of human genome sequencing, the relationship between gene and diet has become a new focus in nutrition research. In epidemiological studies, hypertension and obesity are found to be polygenic diseases. And there is insulin resistance, that is, abnormal insulin signaling pathway. Salt limitation, as an effective non-drug means, plays an active role in controlling blood pressure, obesity and reducing insulin resistance. Based on the above research. In this paper, the effects of low-sodium diet on the expression of genes related to insulin signaling pathway were revealed at the molecular level by using gene chip technology, and a local network of interrelationship between genes was established. The low sodium salt used in this paper is provided by Kawasaki Food Co., Ltd., its NaCl content is about 50%. Under supervision, this salt is used to replace the common salt. And 9 subjects with hypertension and obesity were tested for 12 weeks. Blood samples before and after eating were collected and microarray experiments were carried out. In this paper, 114 genes related to insulin signaling pathway were identified by bioinformatics. Using the 22K human genome microarray of Boo Biological Co., Ltd., the main mutated gene Akt2, Akt3, GSK3, GSK3, GCK3, HK2. And SOS was involved in the related pathways:: PI-3K / Akt Signaling Pathway, Focal Adhesion. T Cell Receptor Signaling Pathway. JAK-STAT Signaling Pathway. for people with high blood pressure and obesity, use a more targeted chip from Kangcheng Bioengineering. Only 114 genes associated with insulin signaling pathway were detected. The main pathways involved are: PI-3K / Akt Signaling Pathway, Focal Adhesion. B Cell Reception Signaling Pathway. Long-term depression.According to the analysis of the local network of these genes, it is found that the changes in the expression of these genes have a certain effect on the transduction of insulin signal-related pathways. Regulation of cell activity and improvement of glucose uptake and transport can reduce insulin resistance to a certain extent. It is hoped that this paper can provide new ideas and clues for salt limitation research.
【学位授予单位】：天津科技大学
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R341

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