碘乙酰胺灌胃联合夹尾应激诱导大鼠FD模型的建立及评价

发布时间：2018-01-30 13:06

本文关键词： 功能性消化不良碘乙酰胺灌胃夹尾应激内脏敏感性胃顺应性胃排空率　出处：《中国中西医结合消化杂志》2015年07期 　论文类型：期刊论文

【摘要】：[目的]采用碘乙酰胺(iodoacetamide,IA)灌胃刺激联合夹尾应激法建立功能性消化不良(functional dyspepsia,FD)大鼠模型,并对其进行初步评价。[方法]18只10日龄SD大鼠随机分为正常组(9只)和模型组(9只)。正常组大鼠每只给予2%蔗糖溶液0.2ml/d灌胃,持续6d;模型组大鼠每只给予0.1%的碘乙酰胺与2%蔗糖的混合液0.2ml/d灌胃,持续6d。灌胃结束后各组大鼠正常饲养至7周龄,7周龄起以郭氏夹尾刺激法对模型组大鼠刺激1周,正常组不予任何干预,直至大鼠8周龄。大鼠8周龄时检测大鼠胃敏感性(以胃扩张刺激时大鼠颈斜方肌肌电均方根变化率为评价指标)及顺应性(以胃扩张刺激时大鼠胃内球囊容积/压力为评价指标),并于术后第二天检测各组大鼠3h摄食量及胃排空率。[结果]在IA灌胃期间(大鼠10日龄至15日龄),模型组大鼠体重增长率较正常组显著降低(P0.05)。大鼠7周龄时,各组大鼠体重无显著差异(P0.05)。7周龄开始予夹尾刺激1周后(大鼠8周龄),模型组大鼠体重较正常组显著降低,结果具有统计学意义(P0.05)。大鼠8周龄时胃敏感性及顺应性检测结果显示:在胃内球囊压力为40-60mmHg时,模型组大鼠与正常组大鼠相比较,颈斜方肌肌电均方根变化率显著升高(P0.05),胃顺应性显著降低(P0.05)。胃排空率结果显示:模型组大鼠胃排空率较正常组大鼠显著降低(P0.05)。[结论]采用IA灌胃法与夹尾应激法相结合的方法建立FD大鼠模型可在一定程度上模拟FD的临床症状及内脏高敏感、胃顺应性降低、胃排空延缓等病理状态。
[Abstract]:[Objective] to establish functional dyspepsia (dyspepsia) by intragastric administration of iodoacetamide iodide combined with tail-clamping stress. FD-) rat model and its preliminary evaluation. [Methods: eighteen 10-day-old SD rats were randomly divided into normal group (n = 9) and model group (n = 9). Normal rats were given 2% sucrose solution 0.2 ml / d intragastrically for 6 days. Rats in the model group were fed with 0.2 ml / d of 0.1% iodide acetamide and 2% sucrose for 6 days. The rats in each group were fed to 7 weeks of age. From the age of 7 weeks, the rats in the model group were stimulated by Guo's clip tail stimulation method for 1 week, but no intervention was given in the normal group. Up to 8 weeks of age, the gastric sensitivities of rats were detected at 8 weeks of age (with the change rate of RMS of the rat cervical trapezius muscle (RMS) as an index) and compliance (. The balloon volume / pressure in the stomach of rats with gastric dilatation was used as the evaluation index. The 3 h intake and gastric emptying rate were measured on the second day after operation. [Results: during the period of intragastric perfusion of IA (10 days to 15 days old rats), the growth rate of body weight in the model group was significantly lower than that in the normal group, and at 7 weeks of age, the growth rate of body weight in the model group was significantly lower than that in the normal group. There was no significant difference in body weight among the three groups. The body weight of the model group was significantly lower than that of the normal group after 1 week of clip tail stimulation (8 weeks old rats). Results the results showed that the gastric sensitivity and compliance of rats at the age of 8 weeks were significantly higher than that of P0.05.The results showed that the gastric balloon pressure was 40-60 mmHg. Compared with normal rats, the change rate of RMS in the model group was significantly higher than that in the normal group (P0.05). Gastric compliance decreased significantly (P 0.05). The gastric emptying rate in the model group was significantly lower than that in the normal group. [Conclusion: the establishment of FD rat model by IA method combined with clip tail stress method can simulate the clinical symptoms and visceral hypersensitivity of FD to some extent, and decrease gastric compliance. Gastric emptying delay and other pathological state.
【作者单位】：北京中医药大学;首都医科大学附属北京中医医院;
【基金】：北京市科委课题:“仁术健脾理气颗粒临床前研究”(No:Z131100002513012) 北京市医院管理局临床医学发展专项经费资助(No:ZYLX201411) 北京市卫生系统高层次卫生技术人才培养计划(No:2011-2-13)
【分类号】：R57;R-332
【正文快照】： 功能性消化不良(functional dyspepsia,FD)是指一组目前尚无器质性原因可究的持续性或反复发作性的消化不良症候群[1],其症状主要包括餐后饱胀不适、上腹胀满、嗳气、腹痛、厌食等,是临床上最常见的功能性胃肠病之一,在全球范围的总患病率为11.5%~14.5%[2]另,有流行病学调查显

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