大鼠实验性糖尿病和脑梗死模型的建立及其指标的检测

发布时间：2018-02-04 04:49

本文关键词： 糖尿病非胰岛素依赖型糖尿病模型:大鼠 GSH-Px SD大鼠大脑中动脉动物模型　出处：《昆明医学院》2008年硕士论文　论文类型：学位论文

【摘要】： 目的建立发病过程近似于人类2型糖尿病,且具有胰岛素抵抗特征的动物模型。探讨此模型与血脂、抗氧化之间的关系。方法8周龄SD雄性大鼠120只,随机分为对照组和造模组。造模组90只雄性SD大鼠喂以富含不饱和脂肪酸的特殊高脂肪膳食4周,诱发出胰岛素抵抗,继之以链脲佐菌素(STZ,25mg/kg)腹腔注射,隔四周第二次40mg/kg剂量腹腔注射,导致胰岛素代偿性分泌障碍,诱发高血糖症;对照组30只雄性SD大鼠用普通饲料喂养。用生化仪器检测血脂,按试剂盒说明测定GSH-Px。结果从生化指标方面证实两次给药法可以成功制备2型糖尿病大鼠模型。糖尿病组模型成模率为72%,死亡率为33%;糖尿病模型的LDL为0.75±0.32mmol/L,TC为1.43±1.07 mmol/L,TG为0.79,1.59,2.04 mmol/L,GHb为6.71±1.37%均较对照组高,GSH-Px较对照组低。结论通过高脂膳食结合STZ注射成功复制出了实验性2型糖尿病动物模型,是研究2型糖尿病发病机制、药物研究和胰岛素抵抗相关疾病的理想动物模型。且此模型的血脂及糖化血红蛋白明显升高,GSH-Px活性会降低。目的糖尿病导致的脑梗死(cerebral infarction,CI)是临床常见病和多发病,具有致残率和病死率高的特点,严重影响着患者的预期寿命和生活质量。近几十年来对脑梗死进行了大量的研究,急性脑梗死的预后比较差。而糖尿病导致的脑梗死损伤的机制十分复杂,缺乏有效的干预手段。因此需要建立良好的糖尿病合并局灶性脑缺血模型。方法采用SD大鼠局部脑缺血模型。随机选取10只SD大鼠分为2组,A1组为假手术组,B1为手术组用改良线栓法;30只SD糖尿病大鼠随机分为2组,A2组为假手术糖尿病组,B2组用改良线栓法为糖尿病手术组,分别采用神经功能缺损评分(NSS)、TTC染色与HE染色动态观察神经功能缺损征、脑梗死体积及观察病理改变情况。四组分别比较模型制作成功率、神经功能缺失评分、脑梗死体积、模型稳定性。结果A1组制作成功率为100%;B1组制作成功率为66.7%;A2组制作成功率为75%,神经功能缺失评分3-4级占75%,脑梗死体积为55.23±9.59mm~3;B2组制作成功率为53.3%,神经功能缺失评分3-4级占60%,脑梗死体积为54.33±6.39mm~3。梗死后大鼠出现不同程度的对侧肢体偏瘫,实验组和对照组所有大鼠术后6h各种评分都处于高水平,各组相比无显著统计学差异,考虑为麻醉药品和手术创伤所致。假手术A1及B1组大鼠在24h左右恢复正常。结论大鼠MCAO模型具有良好的稳定性。适用于急、慢性实验研究,且不需要特殊设备。脑梗死模型比较接近人类脑梗死的病理过程和形态学特点,可模拟形成的缺血对脑组织的损害,是脑梗死的基础研究和治疗干预研究较为理想的动物模型。
[Abstract]:Objective To establish an animal model of insulin resistance in the pathogenesis of type 2 diabetes mellitus , and to explore the relationship between the model and blood lipid and oxidation resistance . Methods 120 male SD rats were randomly divided into control group and module group . 90 male SD rats were fed with special high fat diet rich in unsaturated fatty acid for 4 weeks . Insulin resistance was induced . Results The model of type 2 diabetic rats was successfully prepared from the biochemical indexes . The model of diabetes was 72 % and the mortality rate was 33 % . The LDL of diabetic model was 0.75 卤 0.32 mmol / L , TC was 1.43 卤 1.07 mmol / L , TG was 0 . 79 , 1 . 59 , 2 . 04 mmol / L , GHb was 6.71 卤 1 . 37 % , and that of GSH - Px was lower than that of the control group . Conclusion The experimental animal model of experimental type 2 diabetes is successfully reproduced by high fat diet combined with insulin injection . It is an ideal animal model to study the pathogenesis , drug research and insulin resistance related diseases of type 2 diabetes . The blood lipids and glycosylated hemoglobin of this model are obviously increased , and the activity of GSH - Px is decreased . Objective To study the effect of cerebral infarction ( CI ) on cerebral infarction ( CI ) in patients with diabetes mellitus . Methods Ten SD rats were randomly divided into 2 groups , group A1 as sham operation group and group B2 as sham operation group . Results The success rate of group A1 was 100 % , the success rate of group B1 was 66.7 % , the success rate of group A2 was 75 % , neurological deficit grade 3 - 4 was 75 % , the volume of cerebral infarction was 55.23 卤 9.59mm ~ 3 , the volume of cerebral infarction was 54.33 卤 6.39mm ~ 3 , the volume of cerebral infarction was 54.33 卤 6.39mm ~ 3 , the volume of cerebral infarction was 54.33 卤 6.39mm ~ 3 . Conclusion The rat model has good stability . It is suitable for acute and chronic experimental study , and does not need special equipment . The cerebral infarction model is close to the pathological process and morphological characteristics of human cerebral infarction . It is an ideal animal model for the basic research and therapeutic intervention of cerebral infarction .

【学位授予单位】：昆明医学院
【学位级别】：硕士
【学位授予年份】：2008
【分类号】：R587.1;R743.3;R-332

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