肺移植术后早期移植气管的病理学改变及γδT细胞变化的研究

发布时间：2018-02-04 17:11

本文关键词： 肺移植动物模型固有免疫 γδT细胞 IL-A　出处：《心肺血管病杂志》2015年01期 　论文类型：期刊论文

【摘要】：目的:建立同种同型小鼠原位气管移植模型,模拟并观察肺移植手术早期移植气管的病理学改变和固有γδT细胞的动态变化。方法:雄性C57BL/6小鼠(n=45),随机分为对照组(n=15)和实验组(n=30),各组又随机分为1天、3天、5天三个亚组。对实验组小鼠建立原位气管移植模型,术后第1,3,5天对两组小鼠行肺功能检查、肺泡灌洗液行总细胞计数及分类、移植气管组织病理学检查,使用流式细胞技术检测气管引流淋巴结中γδT细胞的比例,并采用实时荧光定量PCR技术检测移植气管组织中IL-17A基因表达水平。结果:与对照组比较,实验组术后第3,5天的肺总阻力明显增高(P0.05);实验组肺泡灌洗液中总细胞计数均显著增加(P0.05),且以巨噬细胞和中性粒细胞为主;实验组术后气管黏膜下层水肿;实验组术后第1,3天小鼠气管引流淋巴结中γδT细胞占CD3+T细胞的比例明显高于对照组[1天:(2.66±0.19)vs.(1.82±0.13)%;3天:(2.33±0.07)vs.(1.82±0.16)%];与对照组相比,实验组中所有亚组IL-17A mRNA的表达量明显增加(P0.05)。结论:同种同型小鼠气管移植手术创伤,可激活γδT细胞等固有免疫细胞,其中γδT细胞可能通过分泌IL-17A进一步募集巨噬细胞和中性粒细胞,加剧移植术后的炎性反应,导致移植气管黏膜下层水肿。
[Abstract]:Objective: to establish orthotopic trachea transplantation model of homologous mice. The pathological changes of trachea grafts and the dynamic changes of intrinsic 纬未 T cells in the early stage of lung transplantation were simulated and observed. Each group was randomly divided into three subgroups: the control group (n = 15) and the experimental group (n = 30). Each group was randomly divided into three subgroups: one day, three days and five days. The model of orthotopic trachea transplantation was established in the experimental group. The lung function of the two groups was examined in 5 days, the total cell count and classification in alveolar lavage fluid, the histopathological examination of transplanted trachea, and the proportion of 纬未 T cells in the lymph nodes of tracheal drainage were detected by flow cytometry. The expression of IL-17A gene in trachea was detected by real-time fluorescence quantitative PCR. Results: compared with the control group, the experimental group was the third after operation. The total pulmonary resistance increased significantly at 5 days (P 0.05). The total cell count in the alveolar lavage fluid of the experimental group increased significantly (P 0.05), and macrophages and neutrophils were the main ones. Submucosal edema of trachea was observed in the experimental group. The ratio of 纬未 T cells to CD3 T cells in tracheal drainage lymph nodes of the experimental group was significantly higher than that of the control group on the 1st day after operation. [1 day: 2.66 卤0.19 vs.1.82 卤0.13; (3) 2. 33 卤0. 07 vs.1.82 卤0. 16%; Compared with the control group, the expression of IL-17A mRNA in all subgroups of the experimental group was significantly higher than that in the control group. It can activate 纬未 T cells and other innate immune cells, in which 纬未 T cells may further recruit macrophages and neutrophils by secreting IL-17A, thus exacerbating the inflammatory response after transplantation. Causes submucosal edema in the transplanted trachea.
【作者单位】：首都医科大学免疫学系;
【基金】：国家自然科学基金(81370188)
【分类号】：R655.3;R-332
【正文快照】： 肺移植是治疗终末期肺脏疾病的最终手段。移植手术早期可引起固有免疫应答,直接造成器官损伤,进而影响移植器官的长期存活。研究表明γδT细胞在肺部大量存在[1],在固有免疫应答[2]和宿主防御等过程[3-4]均发挥重要作用。因此,研究肺移植术后早期γδT细胞等固有免疫细胞的变

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