辽宁省2006-2012年霍乱弧菌表型特征及分子分型研究

发布时间：2018-02-11 14:18

本文关键词： 霍乱弧菌表型分子分型脉冲场凝胶电泳　出处：《中国微生态学杂志》2015年01期 　论文类型：期刊论文

【摘要】：目的分析辽宁地区2006-2012年霍乱弧菌的表型及分子分型特征。方法采用K-B纸片法,对29株霍乱弧菌菌进行10种抗菌药物的药敏试验;以PCR检测霍乱毒素基因(ctx A)、小带联结毒素基因(zot)、辅助霍乱肠毒素基因(ace)、溶血素基因(hly A)、毒素协调菌毛基因(tcp A)、外膜蛋白基因(omp U)和调控蛋白基因(toxR);采用脉冲场凝胶电泳(PFGE)方法对菌株进行分子分型。结果霍乱弧菌对大部分抗生素敏感。毒力基因检测中,只有2011年水产品来源的菌株含有ctx A基因,水产来源菌株都含有hly A、toxR、omp U;外环境来源菌株均检出hly A、toxR。对29株霍乱弧菌进行PFGE分型(Not I酶切),共分为16个型别,分成4个聚类。结论辽宁省霍乱弧菌随着时间推移表型及分子特征均发生了变化。
[Abstract]:Objective to analyze the phenotypic and molecular typing characteristics of Vibrio cholerae from 2006 to 2012 in Liaoning Province. Methods K-B disk method was used to test the antimicrobial susceptibility of 29 strains of Vibrio cholerae. PCR was used to detect the cholera toxin gene (CTX An), the small band associated toxin gene (TXA), the small band associated toxin gene (Zott), the auxiliary cholerae enterotoxin gene (Aracea), the hemolysin gene (Hly An), the toxin coordinating fimbria gene (TCP Anu), the outer membrane protein gene (OMP UP) and the regulatory protein gene (ToxRX). Results Vibrio cholerae were sensitive to most antibiotics. In 2011, only the strains from aquatic products contained ctx A gene, and all the strains from aquatic products contained hly Atox Romp U. hly Atox Ru was detected from all the strains from external environment. 29 strains of Vibrio cholerae were digested with PFGE type not I enzyme, which were divided into 16 types. Conclusion the phenotypic and molecular characteristics of Vibrio cholerae in Liaoning Province have changed over time.
【作者单位】：辽宁省疾病预防控制中心;
【基金】：艾滋病和病毒性肝炎等重大传染病防治(2012ZX10004209)
【分类号】：R378.3

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