不同地域恒河猴感染H37Rv结核菌株的差异比较

发布时间：2018-02-16 19:16

本文关键词： 中国恒河猴血液学指标血生化指标细菌载量组织病理　出处：《中国病原生物学杂志》2015年08期 　论文类型：期刊论文

【摘要】：目的探讨不同地理分布的恒河猴亚种在结核分枝杆菌感染实验中的反应差异,为在结核疫苗评价中选择合适的动物模型提供依据。方法通过纤维支气管镜定量感染湖北、广西恒河猴,建立急性感染结核病模型。在不同的时间点检测血液、血生化(包括C反应蛋白)、血沉等指标,并对组织细菌载量、肺组织和淋巴结病理切片进行对比分析。结果湖北恒河猴的血液学指标在感染期间均呈下降趋势,在第16、20周(感染后期)均低于广西恒河猴,但血沉值在12、16、20周呈上升趋势,均高于广西恒河猴。两组恒河猴血液中性粒细胞在第12周(湖北猴1.1×1010/L,广西猴4.7×109/L)、淋巴细胞第4周(湖北猴1.7×109/L,广西猴6.8×109/L)和嗜碱性粒细胞在感染前(湖北猴4.3×107/L,广西猴2.0×108/L)差异有统计学意义(P0.01),血小板在16周(湖北猴3.8×1011/L,广西猴4.5×109/L)、20周(湖北猴4.1×1011/L,广西猴4.9×109/L)差异有统计学意义(P0.01)。湖北恒河猴左肺细菌载量(4.52lgCFU/g)低于广西恒河猴(6.30lgCFU/g)(P0.05)。与广西恒河猴相比较,湖北恒河猴左、中、上肺有明显结核结节。结论在MTB的急性感染实验中,湖北和广西恒河猴的血液学指标、部分组织的细菌载量和病理改变均存在差异。
[Abstract]:Objective to explore the difference of the response of different geographical distribution of rhesus monkey subspecies in Mycobacterium tuberculosis infection test, and to provide the basis for selecting suitable animal model in the evaluation of tuberculosis vaccine. In Guangxi rhesus monkey, an acute infection tuberculosis model was established. Blood, blood biochemistry (including C-reactive protein, erythrocyte sedimentation rate, etc.) and bacterial load in tissues were detected at different time points. Results the hematological indexes of Hubei rhesus monkey showed a downward trend during infection, and were lower than those of Guangxi rhesus monkey at the 20th week of infection (late infection), but the ESR showed an increasing trend at 1216 ~ 20 weeks. The blood neutrophils of the two groups were higher than those of the Guangxi rhesus monkeys at the 12th week (1.1 脳 10 10 / L in Hubei, 4.7 脳 10 9 / L in Guangxi, 1.7 脳 10 9 / L in Hubei, 6.8 脳 10 9 / L in Guangxi) and basophil before infection (4.3 脳 10 7 / L in Hubei monkey, 2.0 脳 10 8 / L in Guangxi monkey). There was a statistically significant difference in platelet levels at 16 weeks (3.8 脳 1011 / L in Hubei monkey, 4.5 脳 109 / L / L in Guangxi monkey, 4.1 脳 1011 / L in Hubei monkey and 4.9 脳 109 / L in Guangxi monkey). The bacterial load in left lung of Hubei rhesus monkey was lower than that of Guangxi rhesus monkey (6.30lg CFU P 0.05g). There are obvious tuberculous nodules in the left, middle and upper lungs of Hubei rhesus monkey. Conclusion in the acute infection experiment of MTB, the hematological indexes, bacterial load and pathological changes in some tissues of Hubei and Guangxi rhesus monkeys are different.
【作者单位】：武汉大学动物实验中心/ABSL-3实验室;武汉大学基础医学院人体寄生虫学教研室;
【基金】：国家自然科学基金项目(No.81273010)
【分类号】：R-332;R378

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