肿瘤坏死因子α和白介素6对人髓核细胞凋亡的影响

发布时间：2018-02-23 20:36

  本文关键词： 人髓核细胞 信号传导 炎症因子 P38MAPK JNK/SAPK 细胞凋亡　出处：《青岛大学》2010年硕士论文　论文类型：学位论文

【摘要】： 目的研究炎症因子肿瘤坏死因子α(TNF-α)和白介素6(IL-6)对人髓核细胞凋亡的影响及可能机制。方法培养人髓核细胞,将细胞随即分为7组：TNF-α刺激组；TNF-α+P38MAPK阻断组；TNF-α+JNK/ SAPK阻断组；IL-6刺激组；IL-6+P38MAPK阻断组;IL-6+JNK/SAPK及对照组。然后用AnnexinⅤ/PI染色流式细胞仪和TUNEL法检测髓核细胞凋亡情况,免疫荧光检测P-P38MAPK和P-JNK/SAPK的表达及定位；Western印迹法检测P38MAPK,JNK/SAPK及其磷酸化形式的表达。结果在流式细胞仪检测凋亡结果中,TNF-α和IL-6刺激组相对于对照组及相应阻断组均有着较高的凋亡细胞密度(P＜0.01)；TUNEL法检测凋亡结果中,TNF-α和IL-6刺激组相对于对照组及相应阻断组亦均有着较高的凋亡细胞密度(P0.01)；免疫荧光结果显示TNF-α和IL-6刺激组P-P38MAPK和P-JNK/SAPK在细胞质和细胞核均有表达,各阻断组在细胞质和细胞核可见少量表达,对照组仅见极少量表达,TNF-α和IL-6刺激组差异和相应阻断组及对照组间差异有显著统计学意义(P＜0.01),Western印迹法结果显示P38MAPK,JNK/SAPK在各组髓核细胞内均有表达,但无活化形式,TNF-α和IL-6刺激组可见P-P38MAPK,P-JNK/SAPK表达,但相应阻断组无表达。结论外源性TNF-α和IL-6可通过激活P38MAPK和JNK/SAPK途径导致人髓核细胞凋亡。
[Abstract]:Objective to study the effect of inflammatory factor TNF- 伪 (TNF- 伪) and interleukin-6 (IL-6) on apoptosis of human nucleus pulposus cells. Methods Human nucleus pulposus cells were cultured. The cells were then divided into 7 groups: TNF- 伪 P38MAPK blocking group (TNF- 伪 P38 MAPK blocking group), TNF- 伪 JNK/SAPK blocking group (TNF- 伪 JNK/SAPK blocking group), IL-6 P38 MAPK blocking group (IL-6 JNK/SAPK) and control group (control group). The apoptosis of nucleus pulposus cells was detected by Annexin 鈪，

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