用抗生素根除幽门螺杆菌对食道下端菌群的影响

发布时间：2018-02-24 14:38

本文关键词： 食道下端菌群幽门螺杆菌感染抗生素聚合酶链反应-变性梯度凝胶电泳技术　出处：《重庆医科大学》2014年硕士论文　论文类型：学位论文

【摘要】：目的通过小鼠动物模型，观察幽门螺杆菌（Helicobacter pylori，H. pylori)感染和用抗生素根除治疗后食道下端菌群的变化，探讨其与食道下端疾病发生的关系。方法将45只BALB|C小鼠随机分为阴性对照组，感染组和治疗组。感染组和治疗组通过灌喂幽门螺杆菌菌液建立动物感染模型，治疗组再将奥美拉唑，氨苄青霉素，克拉霉素灌喂根除幽门螺杆菌。三组小鼠均在用抗生素处理后同时处死，取食道下端组织提取细菌的DNA，以原核生物16srDNAV6区通用引物采用聚合酶链反应-变性梯度凝胶电泳技术PCR-DGGE V6区进行扩增，用QuantityOne1-DAnalysis software对DGGE图谱进行菌群分析，并将DGGE图谱上的组间差异条带用16srDNA V6-V8区引物分别扩增后，DNA测序，BLAST比对分析。结果感染组幽门螺杆菌的感染率为100%，，治疗组幽门螺杆菌的根除率为93%；各实验组DGGE指纹图谱分析显示各组间条带数量均有明显的差异(P＜0.05)。三组之间多样性指数、丰富度指数有显著性差异（0.05＞P＞0.01）,均匀度指数无显著差异（P＞0.05）。菌群聚类分析能很好地在相似性进化树中分开，主成分分析不同组的菌群分别聚集在不同的位置，BLAST比对分析对照组、感染组出现特有细菌。结论：食道下端定植着以乳杆菌、拟杆菌为优势菌的稳定菌群，幽门螺杆菌感染后菌群结构变化为以葡萄球菌、不动杆菌、无芽孢杆菌为优势菌的菌群。
[Abstract]:Objective To observe the relationship between Helicobacter pylori ( H . pylori ) infection and the low - end flora of esophagus after the treatment of Helicobacter pylori ( H . pylori ) infection by animal model . Methods 45 BALB / c mice were randomly divided into the negative control group , the infection group and the treatment group . All three groups were sacrificed at the same time after antibiotic treatment . The DNA of bacteria was extracted from the lower end of the feeding passage . The PCR - DGGE V6 region was amplified by polymerase chain reaction - denaturing gradient gel electrophoresis ( PCR - DGGE V6 region ) . The DGGE pattern was analyzed by QuantityOne1 - DAnalysis software . Results The infection rate of Helicobacter pylori in the infected group was 100 % , and the eradication rate of H . pylori in the treatment group was 93 % . There was a significant difference in the number of bands between the three groups ( P < 0.05 ) . Conclusion : The lower end of the esophagus is fixed with the stable flora of Lactobacillus and Pseudobacterium as dominant bacteria , and the structure of the flora after Helicobacter pylori infection changes to the flora of the dominant bacteria with Staphylococcus , non - moving bacillus and bacillus .

【学位授予单位】：重庆医科大学
【学位级别】：硕士
【学位授予年份】：2014
【分类号】：R378

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