人脐带源间充质干细胞生物学特性及移植免疫学研究

发布时间：2018-02-26 17:05

本文关键词： 脐带源间充质干细胞成骨诱导淋巴细胞混合培养细胞因子免疫逃逸免疫应答免疫调节　出处：《吉林大学》2010年博士论文　论文类型：学位论文

【摘要】： Friedenstein等发现的骨髓MSCs有着巨大生物学潜能及应用前景,而其高度污染性及随年龄增加而扩增与分化能力降低、创伤性操作等缺点限制了其广泛使用。2003年HUC-MSCs的成功提取及相关研究证实其具有骨髓MSCs的特点并具有来源充分、提取简单、增殖旺盛、不悖伦理、无创操作等优势。但HUC-MSCs成为骨组织工程学种子细胞面临是否存在同种异体移植排斥问题。全面研究其移植免疫学特性成为HUC-MSCs是否具有临床应用前景的关键。本课题成功对HUC-MSCs进行提取,观察其生物学特征及体外成骨诱导,对诱导体系的变化进行动态观察,检测其不同条件下对同种异体白细胞抗原、B7因子的表达,设定多重因素下的MLR,对临床应用前后及MLR反应前后T细胞亚型及细胞因子变化进行分析,并对其免疫学机制进行探讨,为临床应用提供直接的理论依据。结论:HUC-MSCs为MSCs家族中一员并具有增殖旺盛、传代稳定、体外诱导成骨高效、同种异体移植的免疫逃逸及免疫调节能力,可能成为骨组织工程学理想的种子细胞。
[Abstract]:Bone marrow MSCs discovered by Friedenstein et al has great biological potential and application prospect, and its high pollution, amplification and differentiation ability decrease with age. In 2003, the successful extraction of HUC-MSCs and related studies confirmed that it has the characteristics of bone marrow MSCs and has sufficient source, simple extraction, vigorous proliferation, not contrary to ethics. However, HUC-MSCs is faced with the problem of allograft rejection or not. It is the key to study the immunological characteristics of HUC-MSCs transplantation for its clinical application prospect. In this study, HUC-MSCs was extracted successfully, its biological characteristics and osteogenic induction in vitro were observed, the changes of induction system were observed dynamically, and the expression of allogeneic leukocyte antigen factor B7 was detected under different conditions. The changes of T cell subtypes and cytokines before and after clinical application and MLR reaction were analyzed, and the immunological mechanism was discussed. Conclusion\\\. May be an ideal seed cell for bone tissue engineering.
【学位授予单位】：吉林大学
【学位级别】：博士
【学位授予年份】：2010
【分类号】：R329

【引证文献】