转染HCN2基因的间充质干细胞对兔病窦模型的影响

发布时间：2018-02-27 00:27

本文关键词： 病窦模型超极化激活环核心苷酸门控离子通道生物起搏间充质干细胞射频消融　出处：《华中科技大学》2010年硕士论文　论文类型：学位论文

【摘要】：目的寻找一种能长期稳定的兔病窦模型的制作方法,并检测转染了HCN2基因的间充质干细胞对兔病窦模型的影响。方法日本大耳白兔随机分组,分别用射频消融法和甲醛湿敷法制作逸病窦模型。兔麻醉后经胸骨右缘开胸,大头电极于上腔静脉和右心房交接处窦房结区域放电,以窦性心率下降30-50%或出现交界性逸搏心律为消融成功标志,并且甲醛湿敷法做对照。观察两种方法后兔的心率变化,并分别测其固有心率。体外培养间充质干细胞,提取含HCN2和GFP的质粒,并将质粒转染到间充质干细胞上,注射入病窦兔右心房,对照组用生理盐水注射,观察转染了HCN2基因的间充质干细胞对兔病窦模型的影响。结果射频消融法制作的兔病窦模型较甲醛湿敷法组心率及固有心率均明显减慢,甲醛湿敷法组较与正常组比较亦没有明显差异。注射了含HCN2基因间充质干细胞的兔心率与对照组相比,没有明显差别。结论与常用的甲醛消融法相比,射频消融法制作兔病窦模型是一种更优化的方法。兔右心房不是构建生物起搏器的适宜注射位点。
[Abstract]:Purpose. To find a long-term stable rabbit sinus model and detect the effect of mesenchymal stem cells (MSCs) transfected with HCN2 gene on rabbit sick sinus model. Method. Japanese large ear white rabbits were randomly divided into two groups: radiofrequency ablation method and formaldehyde wet compress method, respectively. After anesthesia, the right sternum was opened and the large head electrode was discharges in the sinus node area at the junction of superior vena cava and right atrium. The sinus heart rate decreased by 30-50% or the borderline escape rhythm was used as the successful sign of ablation, and the formaldehyde wet compress method was used as the control. The changes of heart rate were observed and the intrinsic heart rate was measured respectively. The mesenchymal stem cells were cultured in vitro. The plasmids containing HCN2 and GFP were extracted and transfected into the mesenchymal stem cells (MSCs) and injected into the right atrium of the diseased sinus rabbits. The control group was injected with normal saline to observe the effect of the mesenchymal stem cells (MSCs) transfected with HCN2 gene on the rabbit model of diseased sinus. Results. The heart rate and intrinsic heart rate of the rabbit sinus model made by radiofrequency ablation were significantly slower than those of the wet compress group. The heart rate of rabbits injected with HCN2 gene mesenchymal stem cells was not significantly different from that of the control group. Conclusion. Compared with formaldehyde ablation method, radiofrequency ablation is a better method for the establishment of rabbit sick sinus model, and the right atrium is not a suitable injection site for the construction of biological pacemakers.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2010
【分类号】：R541.7;R-332

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