双组分信号转导系统saeRS对表皮葡萄球菌生存能力的影响

发布时间：2018-02-28 00:28

本文关键词： 葡萄糖代谢双组分信号转导系统saeRS 生物膜形成表皮葡萄球菌　出处：《四川大学学报(医学版)》2015年01期 　论文类型：期刊论文

【摘要】：目的研究不同浓度的葡萄糖对表皮葡萄球菌野生株、saeRS基因删除突变株及回复株生存能力的影响及机制。方法在BM培养基中添加不同浓度(7.0mmol/L、14mmol/L、28mmol/L、56mmol/L)的葡萄糖,分析3种表皮葡萄球菌的生存能力、生物膜形成能力、培养基的酸度及抵抗抗生素生存的能力。结果与表皮葡萄球菌野生株相比,葡萄糖能明显促进saeRS删除突变株的生存能力和生物膜形成能力,但能够明显降低其对抗生素(比如青霉素、苯唑西林、庆大霉素、环丙沙星和丁胺卡那霉素)的抵抗能力;saeRS删除突变株和回复株在14mmol/L浓度时生存能力最强,在28mmol/L浓度时生物膜形成能力最强,而葡萄糖浓度对于野生株的生存能力及生物膜形成能力无显著影响。在添加14mmol/L葡萄糖时,saeRS删除突变株培养基(pH=8.07)的酸度比野生株(pH=7.0)明显降低。结论 SaeRS可能通过介导葡萄糖的利用影响了表皮葡萄球菌的生存能力;双组分信号转导系统saeRS影响抗生素如青霉素、苯唑西林、庆大霉素、环丙沙星和丁胺卡那霉素对表皮葡萄球菌的杀菌作用。
[Abstract]:Objective to study the effect and mechanism of glucose concentration on the survival ability of staphylococcus epidermidis wild strain (S.epidermidis) to delete the mutant gene and to recover the gene. Methods glucose of 7.0mmol / L ~ (14) mmol / L ~ (28) mmol / L ~ (28) mmol / L ~ (56) mmol 路L ~ (-1) was added to BM medium. The survival ability, biofilm forming ability, acidity of culture medium and survival ability of antibiotic resistance of three species of Staphylococcus epidermidis were analyzed. Results compared with the wild strains of Staphylococcus epidermidis, Glucose significantly increased the viability and biofilm formation of saeRS deletion mutants, but significantly reduced its resistance to antibiotics (such as penicillin, oxacillin, gentamicin, etc.). The resistance of ciprofloxacin and amikacin) was the strongest at 14 mmol / L and biofilm formation at 28 mmol / L. However, glucose concentration had no significant effect on the viability and biofilm formation ability of wild plants. The acidity of the culture medium containing 14mmol / L glucose was significantly lower than that of wild plants. Conclusion the pH value of SaeRS may be decreased by adding 14mmol / L glucose to the culture medium (pH = 8.07). Conclusion the results suggest that the concentration of glucose has no significant effect on the viability and biofilm formation of wild plants. The utilization of glucose conduction affected the viability of Staphylococcus epidermidis. Two-component signal transduction system (saeRS) affects the bactericidal effects of antibiotics such as penicillin, oxacillin, gentamicin, ciprofloxacin and amikacin on Staphylococcus epidermidis.
【作者单位】：河南大学医学院河南省医学与分子免疫学实验室抗体药物河南省工程实验室;复旦大学上海医学院教育部/卫生部医学分子病毒学重点实验室;
【分类号】：R378.1

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