K1型肺炎克雷伯菌毒力基因及分子分型研究

发布时间：2018-02-28 16:16

本文关键词： 肺炎克雷伯菌 K 毒力基因分子分型 ST　出处：《浙江预防医学》2015年12期 　论文类型：期刊论文

【摘要】：目的研究荚膜多糖分型K1型肺炎克雷伯菌的毒力基因及其分子分型情况。方法收集2013年3—12月期间浙江大学医学院附属第二医院健康体检中心429份健康体检者粪便标本,按不同年龄组计算肺炎克雷伯菌肠道分离率;"拉丝"试验检测高产黏液菌株;聚合酶链反应(PCR)扩增K1、K2、K5、K20、K54、K57、rmp A、mag A和wca G;K-B纸片法检测K1型菌株药物敏感性;采用多位点序列分型(MLST)对K1型肺炎克雷伯菌进行分型。结果粪便标本肺炎克雷伯菌分离率为22.61%。97株肺炎克雷伯菌中"拉丝"阳性率为39.18%。共检测到11株携带K1毒力基因的肺炎克雷伯菌,4株同时携带mag A、wca G和rmp A,9株同时携带wca G和rmp A。12岁组中未发现毒力基因存在。药敏结果显示K1型菌株除对氨苄西林耐药以外,对碳青霉烯类、喹诺酮类和头孢菌素类等抗生素均敏感。11株K1型菌株中有7株"拉丝"试验阳性。K1型菌株MLST分型结果,除1株为ST600外,其他均为ST23。结论粪便中分离的K1型肺炎克雷伯菌往往同时携带毒力基因rmp A、mag A和wca G,对临床常用抗菌药物敏感,ST23为其主要的临床流行株。
[Abstract]:Objective to study the virulence gene and molecular typing of Klebsiella pneumoniae K1 by capsular polysaccharide typing. Methods during the period from March to December of 2013, 429 fecal specimens were collected from the health examination center of the second affiliated Hospital of Zhejiang University Medical College. The intestinal isolation rate of Klebsiella pneumoniae was calculated according to different age groups, the high yield mucus strains were detected by "wire drawing" test, the susceptibility of K1 strain K1 was detected by polymerase chain reaction (PCR) amplification of K1 K2K2K5, K20K5A7rmp Amig A and wca GUK-B disk method. Results the isolation rate of Klebsiella pneumoniae from fecal samples was 22.61.97 strains of Klebsiella pneumoniae were detected, and the positive rate of pulling silk was 39.18.The results showed that 11 strains of Klebsiella pneumoniae carrying K1 virulence were detected. Four strains of Klebsiella pneumoniae were found to carry mag AwcaG and rmp Acanine at the same time. No virulence genes were found in both wca G and rmp A.12 years old group. The results of drug sensitivity showed that K1 strains were resistant to ampicillin, except ampicillin. Seven of 11 K1 strains were sensitive to carbapenes, quinolones and cephalosporins. The results of MLST typing showed that 7 of the 11 K1 strains were positive for "pulling wire" test, except one strain was ST600. Conclusion K1 Klebsiella pneumoniae isolated from feces is usually carrying virulence genes rmp Amomag A and wca G, and ST23 is the main clinical epidemic strain of Klebsiella pneumoniae isolated from feces.
【作者单位】：桐庐县人民医院检验科;温州医科大学;
【分类号】：R378.996

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