骨质疏松肾阳虚病证结合模型构建的实验研究

发布时间：2018-03-01 15:10

本文关键词： 骨质疏松肾阳虚病证结合动物模型　出处：《中国中医急症》2015年02期 　论文类型：期刊论文

【摘要】：目的研究建立骨质疏松肾阳虚病证结合模型的方法。方法雌性昆明小鼠随机分为对照组(A组)、证型模型组(B组)、疾病模型组(C组)、疾病证型模型组(D组)。分别应用去双侧卵巢法构建骨质疏松模型,氢化可的松皮下注射诱导肾阳虚证模型。通过观察小鼠症状体征、促肾上腺皮质激素、促甲状腺激素、环磷酸腺苷、环磷酸鸟苷、骨密度、骨组织形态学等评价模型。结果与A组比较,D组小鼠出现毛发干枯,畏寒倦卧,肛温降低等肾阳虚症状,促肾上腺皮质激素及促甲状腺激素含量降低(P0.01),环磷酸腺苷/环磷酸鸟苷降低(P0.01),股骨近端、腰椎骨密度下降(P0.01),HE染色及骨组织形态计量学分析显示骨小梁排列稀疏、变细,破骨细胞数增多等。结论采取双侧卵巢切除结合氢化可的松皮下注射的方法,能成功构建骨质疏松肾阳虚病证结合模型。
[Abstract]:Objective to study the method of establishing a model of osteoporosis combined with kidney yang deficiency syndrome. Methods female Kunming mice were randomly divided into three groups: control group A, syndrome model group B, disease model group C, disease syndrome model group D. Osteoporosis model was established by bilateral ovariectomy. The model of kidney yang deficiency was induced by subcutaneous injection of hydrocortisone. The symptoms and signs of mice were observed, including adrenocorticotropic hormone, thyroid stimulating hormone, cyclic adenosine monophosphate, cyclic guanosine phosphate, bone mineral density. Results compared with group A, the mice in group D developed symptoms of deficiency of kidney yang, such as dry hair, cold and cold, lower anal temperature, and so on. The contents of adrenocorticotropic hormone (ACTH) and thyroid stimulating hormone (TSH) decreased P0.01a, cyclic adenosine monophosphate / cyclic guanosine monophosphate decreased P0.01a, proximal femur, lumbar bone mineral density decreased P0.01A HE staining and bone histomorphometry analysis showed that bone trabecular arrangement was sparse and thin. Conclusion bilateral ovariectomies combined with subcutaneous injection of hydrocortisone can successfully establish a combined model of osteoporosis with deficiency of kidney yang.
【作者单位】：浙江省绍兴市中医院;
【基金】：浙江省中医药科技计划项目(2011ZB153)
【分类号】：R-332

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