压力性尿失禁动物模型的建立及相关因子（Tgf β-3,Lamin,Relaxin）的检测

发布时间：2018-03-04 21:19

  本文选题：压力性尿失禁　切入点：动物模型　出处：《山西医科大学》2009年硕士论文　论文类型：学位论文

【摘要】： 1.研究目的 1.1建立压力性尿失禁的动物模型; 1.2研究转化生长因子β-3(Tgfβ-3),层粘连蛋白(Lamin),松弛素(Relaxin)在SUI大鼠和正常大鼠阴道前壁和肛提肌组织中的表达情况; 1.3分析三种因子含量在压力性尿失禁动物模型组和正常对照组中表达含量的变化,初步探讨该模型形成的机制以及三种因子与压力性尿失禁发生发展的关系,为临床发病机制、诊断和治疗提供一定的实验室依据。 2.实验方法 2.1将50只雌性SD大鼠分为五组:Ⅰ组为正常对照组(10只);Ⅱ组为正常分娩组(10只);Ⅲ组为正常分娩+阴道扩张组(10只);Ⅳ组为正常分娩+卵巢切除组(10只);Ⅴ组为正常分娩+阴道扩张+卵巢切除组(10只); 2.2进行尿动力学检测,以证实压力性尿失禁; 2.3实验结束后,取实验证实的SUI大鼠与正常大鼠的阴道前壁和肛提肌,RT-PCR分别检测大鼠阴道前壁和肛提肌中三种因子Tgfβ-3、Lamin和Relaxin mRNA含量的表达,从而比较压力性尿失禁动物模型组和正常组中三种因子含量表达的变化。 3.实验结果 3.1 4个月中共4只大鼠死亡,Ⅰ组腹漏尿点压(ALPP)为(74.24±5.01) cmH2O,Ⅱ组ALPP为(70.81±3.06)cm H2O,Ⅲ组ALPP为(52.62±3.63)cm H2O,Ⅳ组ALPP为(53.26±3.45)cmH2O,Ⅴ组ALPP为(35.20±3.61)cmH2O。 3.2Ⅱ组的ALPP低于Ⅰ组,但二者比较无统计学意义(P0.05);Ⅲ组、Ⅳ组和Ⅴ组的ALPP均低于Ⅰ组,两两比较有统计学意义(P0.05);Ⅲ组、Ⅳ组两者ALPP比较无统计学意义(P0.05);Ⅱ组、Ⅲ组、Ⅳ组与Ⅴ组ALPP两两相比差异均有统计学意义(P0.05)。 3.3Ⅴ组中的SUI模型鼠阴道前壁中转化生长因子β-3(Tgfβ-3),层粘连蛋白(Lamin)二种因子的表达含量较Ⅰ组有明显的降低,差异有统计学意义(P0.05);松弛素(Relaxin)因子的表达含量较Ⅰ组有明显的升高,差异有统计学意义(P0.05)。 3.4Ⅴ组中SUI模型鼠肛提肌中转化生长因子β-3(Tgfβ-3),层粘连蛋白(Lamin)二种因子的表达含量较Ⅰ组有明显的降低,差异有统计学意义(P0.01);松弛素(Relaxin)因子的表达含量较Ⅰ组有明显的升高,差异有统计学意义(P0.01)。 4.结论 4.1模拟妊娠、难产、雌激素减退可以成功建立压力性尿失禁动物模型,同时模拟几种病因成功率更高。 4.2压力性尿失禁动物模型中阴道前壁组织中转化生长因子β-3(Tgfβ-3),层粘连蛋白(Lamin)二种因子的表达含量较正常无压力性尿失禁组有明显的降低,松弛素(Relaxin)表达含量有明显的升高。 4.3压力性尿失禁动物模型中肛提肌中转化生长因子β-(3Tgfβ-3),层粘连蛋白(Lamin)二种因子的表达含量较正常无压力性尿失禁组有明显的降低,松弛素(Relaxin)表达含量有明显的升高。提示这Tgfβ-3 ,Lamin,Relaxin与SUI发展有相关性。
[Abstract]:1. purpose of research
1.1 animal model of stress urinary incontinence was established.
1.2, we studied the expression of transforming growth factor beta -3 (Tgf beta -3), laminin (Lamin) and relaxin (Relaxin) in the anterior vaginal wall and the levator ani muscle of SUI rats and normal rats.
1.3 factor analysis of three kinds of content changes in expression of urinary stress incontinence animal model group and normal control group, to investigate the relationship between the formation mechanism of this model and three kinds of factors and stress urinary incontinence development, clinical pathogenesis, and provide laboratory evidence for a diagnosis and treatment.
2. experimental method
2.1 50 female SD rats were randomly divided into five groups: normal control group (10 rats); group II, normal delivery group (10 rats); group III normal delivery + colpectasis group (10 rats); group IV normal delivery + ovariectomy group (10 rats); V group of normal delivery + colpectasis + ovariectomized group (10 rats);
2.2 the urodynamic test was carried out to confirm the pressure urinary incontinence.
2.3 after the end of the experiment, the experiment proved SUI rats and normal rats anterior vaginal wall and levator ani muscle, RT-PCR was used to detect the anterior wall of vagina and anus levator muscle of rats in three kinds of factor Tgf beta -3, Lamin expression and Relaxin content of mRNA, so as to compare group urinary stress incontinence animal model and three a factor in the changes of the expression of the normal group.
3. experimental results
In 3.14 months, 4 rats died. The abdominal leak point pressure (ALPP) in group I was (74.24 + 5.01) cmH2O, group ALPP was (70.81 + 3.06) cm H2O, group III ALPP (52.62 + 3.63) cm H2O, group IV ALPP (53.26 3.45 3.45) cmH2O, group V ALPP (35.20 + 35.20) cmH2O.
The 3.2 group II ALPP lower than that of group I, but there was no significant difference between the two (P0.05); group III, IV and V group group ALPP were lower than that in group I, 22 were statistically significant (P0.05); group III, IV group two ALPP was not statistically significant (P0.05); group II, group III in group IV, V and 22 ALPP group had a significant difference (P0.05).
3.3鈪ょ粍涓�鐨凷UI妯″瀷榧犻槾閬撳墠澹佷腑杞�鍖栫敓闀垮洜瀛愇�-3(Tgf尾-3),灞傜矘杩炶泲鐧，

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