人类软骨糖蛋白39诱导关节软骨祖母细胞的成软骨分化

发布时间：2018-03-05 18:38

  本文选题：软骨细胞　切入点：糖蛋白类　出处：《中国组织工程研究》2015年42期 　论文类型：期刊论文

【摘要】：背景:有研究表明人类软骨糖蛋白39与骨关节软骨的退变与修复具有一定的关系,但其具体的作用机制并不十分明确。目的:观察人类软骨糖蛋白39对成人膝关节软骨祖母细胞成软骨诱导分化的影响。方法:取成人关节软骨,消化分离培养关节软骨祖母细胞;流式细胞仪检测传代细胞中能够表达CD105、CD166的细胞量并进行分离提纯。将分离的软骨祖母细胞采用单层培养法培养,传代培养至第2代后向分离培养所得的软骨祖母细胞中,分别经过含人类软骨糖蛋白39成软骨培养基及普通成软骨诱导培养基的诱导培养14 d后,通过免疫组织化学染色观察经诱导后细胞中Ⅱ型胶原的表达及通过大体组织学观察评估软骨的形成。结果与结论:关节软骨组织中可以分离出能够表达CD105、CD166的关节软骨祖母细胞,软骨祖母细胞经过诱导分化后逐渐聚集并形成结节,经诱导后Ⅱ型胶原免疫细胞化学着色阳性,且经人类软骨糖蛋白39诱导细胞形成的结节更大,Ⅱ型胶原表达更多。结果表明,成人关节软骨中能够分离培养出具有成软骨分化能力的干细胞系细胞即软骨祖母细胞,且能够被定向诱导分化为软骨细胞,人类软骨糖蛋白39对其分化过程具有一定的促进作用。
[Abstract]:Background: studies have shown that human cartilage glycoprotein 39 has a certain relationship with the degeneration and repair of osteoarticular cartilage. Objective: to observe the effect of human chondroglycoprotein 39 on the differentiation of articular cartilage cells induced by human knee joint grandmother chondrocytes. Methods: adult articular cartilage was obtained. Digestion, isolation and culture of articular cartilage grandmother cells; flow cytometry analysis of the amount of cells capable of expressing CD105 and CD166 in the passage cells; isolation and purification of the isolated chondrograndmother cells. The isolated chondrograndmother cells were cultured by monolayer culture. After subculture to the second generation, the chondrograndmother cells were cultured in the cartilage medium containing human chondroglycoprotein 39 and common cartilage induction medium for 14 days, respectively. The expression of type 鈪，

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